Clinically Compliant Spatial and Temporal Imaging of Chimeric Antigen Receptor T-cells

Nia Emami-Shahri, Julie Foster, Roxana Kashani, Patrycja Gazinska, Celia Cook, John Maher, Jane Sosabowski, Sophie Papa

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)
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Abstract

The unprecedented efficacy of chimeric antigen receptor (CAR) T-cell immunotherapy of CD19+ B-cell malignancy has established a new therapeutic pillar of hematology-oncology. Nonetheless, formidable challenges remain for the attainment of comparable success in patients with solid tumors. To accelerate progress and rapidly characterize emerging toxicities, systems that permit the repeated and non-invasive assessment of CAR T-cell bio-distribution would be invaluable. An ideal solution would entail the use of a non-immunogenic reporter that mediates specific uptake of an inexpensive, non-toxic and clinically established imaging tracer by CAR T-cells. Here we show the utility of the human sodium iodide symporter (hNIS) for the temporal and spatial monitoring of CAR T-cell behavior in a cancer-bearing host. This system provides a clinically compliant toolkit for high-resolution serial imaging of CAR T-cells in vivo, addressing a fundamental unmet need for future clinical development in the field.
Original languageEnglish
Article number1081
Number of pages12
JournalNature Communications
Volume9
Issue number1
Early online date14 Mar 2018
DOIs
Publication statusPublished - 14 Mar 2018

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