TY - JOUR
T1 - Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss)
AU - Bussolaro, Daniel
AU - Wright, Stephanie L
AU - Schnell, Sabine
AU - Schirmer, Kristin
AU - Bury, Nicolas R
AU - Arlt, Volker M
N1 - Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Microscopic plastic (MP) particles are a ubiquitous contaminant in aquatic environments, which may bind hydrophobic chemicals, such as polycyclic aromatic hydrocarbons (PAHs), altering their environmental fate and interactions with biota. Using rainbow trout gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells we investigated the effects of polystyrene microbeads (PS-MBs; 220 nm) on the cyto- and genotoxicity of the environmental pollutants benzo[a]pyrene (BaP) and 3-nitrobenzanthrone (3-NBA) over 48 h (0, 0.1, 1 and 10 μM). The Alamar Blue bioassay, used to assess cytotoxicity, showed that both pollutants significantly decreased cell viability by 10-20% at 10 μM in both cell lines after 48 h whereas PS-MBs (5 or 50 μg mL-1) were non-toxic. Cytotoxicity in cells treated with PS-MBs together with BaP or 3-NBA were similar to those observed after exposure to BaP or 3-NBA alone. Using the formamidopyrimidine-DNA glycosylase (FPG)-modified comet assay 3-NBA, but not BaP, induced DNA damage in RTgutGC cells at 10 μM (∼10% tail DNA in the absence and ∼15% tail DNA in the presence of FPG versus ∼1% in controls), whereas PS-MBs alone showed no detrimental effects. Interestingly, comet formation was substantially increased (∼4-fold) when RTgutGC cells were exposed to PS-MBs (50 μg mL-1) and 10 μM 3-NBA compared to cells treated with 3-NBA alone. Further, using 32P-postlabelling we observed strong DNA adduct formation in 3-NBA-exposed RTgutGC cells (∼900 adducts/108 nucleotides). 3-NBA-derived DNA adduct formation was significantly decreased (∼20%) when RTgutGC cells were exposed to MB and 3-NBA compared to cells treated with 3-NBA alone. Our results show that PS-MBs impact on the genotoxicity of 3-NBA, causing a significant increase in DNA damage as measured by the comet assay in the intestinal cell line, providing proof of principle that MPs may alter the genotoxic potential of PAHs in fish cells.
AB - Microscopic plastic (MP) particles are a ubiquitous contaminant in aquatic environments, which may bind hydrophobic chemicals, such as polycyclic aromatic hydrocarbons (PAHs), altering their environmental fate and interactions with biota. Using rainbow trout gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells we investigated the effects of polystyrene microbeads (PS-MBs; 220 nm) on the cyto- and genotoxicity of the environmental pollutants benzo[a]pyrene (BaP) and 3-nitrobenzanthrone (3-NBA) over 48 h (0, 0.1, 1 and 10 μM). The Alamar Blue bioassay, used to assess cytotoxicity, showed that both pollutants significantly decreased cell viability by 10-20% at 10 μM in both cell lines after 48 h whereas PS-MBs (5 or 50 μg mL-1) were non-toxic. Cytotoxicity in cells treated with PS-MBs together with BaP or 3-NBA were similar to those observed after exposure to BaP or 3-NBA alone. Using the formamidopyrimidine-DNA glycosylase (FPG)-modified comet assay 3-NBA, but not BaP, induced DNA damage in RTgutGC cells at 10 μM (∼10% tail DNA in the absence and ∼15% tail DNA in the presence of FPG versus ∼1% in controls), whereas PS-MBs alone showed no detrimental effects. Interestingly, comet formation was substantially increased (∼4-fold) when RTgutGC cells were exposed to PS-MBs (50 μg mL-1) and 10 μM 3-NBA compared to cells treated with 3-NBA alone. Further, using 32P-postlabelling we observed strong DNA adduct formation in 3-NBA-exposed RTgutGC cells (∼900 adducts/108 nucleotides). 3-NBA-derived DNA adduct formation was significantly decreased (∼20%) when RTgutGC cells were exposed to MB and 3-NBA compared to cells treated with 3-NBA alone. Our results show that PS-MBs impact on the genotoxicity of 3-NBA, causing a significant increase in DNA damage as measured by the comet assay in the intestinal cell line, providing proof of principle that MPs may alter the genotoxic potential of PAHs in fish cells.
KW - Fish cell lines
KW - Genotoxicity
KW - Microplastic beads
KW - Polycyclic aromatic hydrocarbons
KW - RTgill-W1
KW - RTgutGC
UR - http://www.scopus.com/inward/record.url?scp=85062717627&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2019.02.066
DO - 10.1016/j.envpol.2019.02.066
M3 - Article
C2 - 30849588
SN - 0269-7491
VL - 248
SP - 706
EP - 714
JO - Environmental pollution (Barking, Essex : 1987)
JF - Environmental pollution (Barking, Essex : 1987)
ER -