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Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production

Research output: Contribution to journalArticle

Xia Dong, Weiju Wu, Liang Ma, Chengfei Liu, Mohajeet B. Bhuckory, Liping Wang, Emeline F. Nandrot, Heping Xu, Ke Li, Yizhi Liu, Wuding Zhou

Original languageEnglish
Pages (from-to)529-545
JournalJournal of innate immunity
Early online date4 Aug 2017
DOIs
Publication statusE-pub ahead of print - 4 Aug 2017

King's Authors

Abstract

In this paper, we report previously unknown roles for collectin-11 (CL-11, a soluble C-type lectin) in modulating the retinal pigment epithelial (RPE) cell functions of phagocytosis and cytokine production. We found that CL-11 and its carbohydrate ligand are expressed in both the murine and human neural retina; these resemble each other in terms of RPE and photoreceptor cells. Functional analysis of murine RPE cells showed that CL-11 facilitates the opsonophagocytosis of photoreceptor outer segments and apoptotic cells, and also upregulates IL-10 production. Mechanistic analysis revealed that calreticulin on the RPE cells is required for CL-11-mediated opsonophagocytosis whereas signal-regulatory protein α and mannosyl residues on the cells are involved in the CL-11-mediated upregulation of IL-10 production. This study is the first to demonstrate the role of CL-11 and the molecular mechanisms involved in modulating RPE cell phagocytosis and cytokine production. It provides a new insight into retinal health and disease and has implications for other phagocytic cells.

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