King's College London

Research portal

Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes

Research output: Contribution to journalArticlepeer-review

Standard

Combined AFM and super-resolution localisation microscopy : Investigating the structure and dynamics of podosomes. / Hirvonen, Liisa M.; Marsh, Richard J.; Jones, Gareth E. et al.

In: European Journal of Cell Biology, Vol. 99, No. 7, 151106, 09.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Hirvonen, LM, Marsh, RJ, Jones, GE & Cox, S 2020, 'Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes', European Journal of Cell Biology, vol. 99, no. 7, 151106. https://doi.org/10.1016/j.ejcb.2020.151106

APA

Hirvonen, L. M., Marsh, R. J., Jones, G. E., & Cox, S. (2020). Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes. European Journal of Cell Biology, 99(7), [151106]. https://doi.org/10.1016/j.ejcb.2020.151106

Vancouver

Hirvonen LM, Marsh RJ, Jones GE, Cox S. Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes. European Journal of Cell Biology. 2020 Sep;99(7). 151106. https://doi.org/10.1016/j.ejcb.2020.151106

Author

Hirvonen, Liisa M. ; Marsh, Richard J. ; Jones, Gareth E. et al. / Combined AFM and super-resolution localisation microscopy : Investigating the structure and dynamics of podosomes. In: European Journal of Cell Biology. 2020 ; Vol. 99, No. 7.

Bibtex Download

@article{776814a5d4cd4baeb848d757e72656b7,
title = "Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes",
abstract = "Podosomes are mechanosensitive attachment/invasion structures that form on the matrix-adhesion interface of cells and protrude into the extracellular matrix to probe and remodel. Despite their central role in many cellular processes, their exact molecular structure and function remain only partially understood. We review recent progress in molecular scale imaging of podosome architecture, including our newly developed localisation microscopy technique termed HAWK which enables artefact-free live-cell super-resolution microscopy of podosome ring proteins, and report new results on combining fluorescence localisation microscopy (STORM/PALM) and atomic force microscopy (AFM) on one setup, where localisation microscopy provides the location and dynamics of fluorescently labelled podosome components, while the spatial variation of stiffness is mapped with AFM. For two-colour localisation microscopy we combine iFluor-647, which has previously been shown to eliminate the need to change buffer between imaging modes, with the photoswitchable protein mEOS3.2, which also enables live cell imaging.",
keywords = "AFM, Localisation microscopy, Multi-modal microscopy, Podosome, Super-resolution",
author = "Hirvonen, {Liisa M.} and Marsh, {Richard J.} and Jones, {Gareth E.} and Susan Cox",
year = "2020",
month = sep,
doi = "10.1016/j.ejcb.2020.151106",
language = "English",
volume = "99",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "7",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Combined AFM and super-resolution localisation microscopy

T2 - Investigating the structure and dynamics of podosomes

AU - Hirvonen, Liisa M.

AU - Marsh, Richard J.

AU - Jones, Gareth E.

AU - Cox, Susan

PY - 2020/9

Y1 - 2020/9

N2 - Podosomes are mechanosensitive attachment/invasion structures that form on the matrix-adhesion interface of cells and protrude into the extracellular matrix to probe and remodel. Despite their central role in many cellular processes, their exact molecular structure and function remain only partially understood. We review recent progress in molecular scale imaging of podosome architecture, including our newly developed localisation microscopy technique termed HAWK which enables artefact-free live-cell super-resolution microscopy of podosome ring proteins, and report new results on combining fluorescence localisation microscopy (STORM/PALM) and atomic force microscopy (AFM) on one setup, where localisation microscopy provides the location and dynamics of fluorescently labelled podosome components, while the spatial variation of stiffness is mapped with AFM. For two-colour localisation microscopy we combine iFluor-647, which has previously been shown to eliminate the need to change buffer between imaging modes, with the photoswitchable protein mEOS3.2, which also enables live cell imaging.

AB - Podosomes are mechanosensitive attachment/invasion structures that form on the matrix-adhesion interface of cells and protrude into the extracellular matrix to probe and remodel. Despite their central role in many cellular processes, their exact molecular structure and function remain only partially understood. We review recent progress in molecular scale imaging of podosome architecture, including our newly developed localisation microscopy technique termed HAWK which enables artefact-free live-cell super-resolution microscopy of podosome ring proteins, and report new results on combining fluorescence localisation microscopy (STORM/PALM) and atomic force microscopy (AFM) on one setup, where localisation microscopy provides the location and dynamics of fluorescently labelled podosome components, while the spatial variation of stiffness is mapped with AFM. For two-colour localisation microscopy we combine iFluor-647, which has previously been shown to eliminate the need to change buffer between imaging modes, with the photoswitchable protein mEOS3.2, which also enables live cell imaging.

KW - AFM

KW - Localisation microscopy

KW - Multi-modal microscopy

KW - Podosome

KW - Super-resolution

UR - http://www.scopus.com/inward/record.url?scp=85088785139&partnerID=8YFLogxK

U2 - 10.1016/j.ejcb.2020.151106

DO - 10.1016/j.ejcb.2020.151106

M3 - Article

AN - SCOPUS:85088785139

VL - 99

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 7

M1 - 151106

ER -

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454