Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production

David Simper; Ursula Mayr; Carmen Urbich; Anna Zampetaki; Marianna Prokopi; Athanasios Didangelos; Angelika Saje; Michael Mueller; Ulrike Benbow; Andrew C. Newby; Rolf Apweiler; Salman Rahman; Stefanie Dimmeler; Qingbo Xu; Manuel Mayr

doi:10.1161/ATVBAHA.110.204651

Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production

David Simper, Ursula Mayr, Carmen Urbich, Anna Zampetaki, Marianna Prokopi, Athanasios Didangelos, Angelika Saje, Michael Mueller, Ulrike Benbow, Andrew C. Newby, Rolf Apweiler, Salman Rahman, Stefanie Dimmeler, Qingbo Xu, Manuel Mayr

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Objective-Recent studies on cardiovascular progenitors have led to a new appreciation that paracrine factors may support the regeneration of damaged tissues. Methods and Results-We used a shotgun proteomics strategy to compare the secretome of peripheral blood-derived smooth muscle progenitors (SPCs) with human aortic smooth muscle cells. The late-outgrowth SPCs produced fewer proteolytic enzymes and inflammatory cytokines and showed reduced invasive capacity. Similar to smooth muscle cells, SPCs secreted extracellular matrix. However, SPCs produced different matrix proteins, as evidenced by the truncation of proangiogenic domains in collagen alpha-1 (I) and increased production of periostin. Moreover, SPCs retained serum proteins, including proteoglycans, regulating collagen assembly; and pigment epithelium-derived factor, a potent inhibitor of angiogenesis. As a functional consequence, their conditioned medium was less angiogenic, as demonstrated by endothelial tube formation assays in vitro and implantation of Matrigel plugs into nude, severe combined immunodeficient mice (NOD/SCID). Conclusion-The present study represents an important conceptual development, suggesting that SPCs may contribute to extracellular matrix production. (Arterioscler Thromb Vasc Biol. 2010; 30: 1325-1332.)

Original language	English
Pages (from-to)	1325 - U127
Number of pages	8
Journal	Arteriosclerosis, Thrombosis, and Vascular Biology
Volume	30
Issue number	7
DOIs	https://doi.org/10.1161/ATVBAHA.110.204651
Publication status	Published - Jul 2010

Access to Document

10.1161/ATVBAHA.110.204651

Cite this

Simper, D., Mayr, U., Urbich, C., Zampetaki, A., Prokopi, M., Didangelos, A., Saje, A., Mueller, M., Benbow, U., Newby, A. C., Apweiler, R., Rahman, S., Dimmeler, S., Xu, Q., & Mayr, M. (2010). Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production. Arteriosclerosis, Thrombosis, and Vascular Biology, 30(7), 1325 - U127. https://doi.org/10.1161/ATVBAHA.110.204651

@article{7699dbda38664edc958ab89443c9c9f5,

title = "Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production",

abstract = "Objective-Recent studies on cardiovascular progenitors have led to a new appreciation that paracrine factors may support the regeneration of damaged tissues. Methods and Results-We used a shotgun proteomics strategy to compare the secretome of peripheral blood-derived smooth muscle progenitors (SPCs) with human aortic smooth muscle cells. The late-outgrowth SPCs produced fewer proteolytic enzymes and inflammatory cytokines and showed reduced invasive capacity. Similar to smooth muscle cells, SPCs secreted extracellular matrix. However, SPCs produced different matrix proteins, as evidenced by the truncation of proangiogenic domains in collagen alpha-1 (I) and increased production of periostin. Moreover, SPCs retained serum proteins, including proteoglycans, regulating collagen assembly; and pigment epithelium-derived factor, a potent inhibitor of angiogenesis. As a functional consequence, their conditioned medium was less angiogenic, as demonstrated by endothelial tube formation assays in vitro and implantation of Matrigel plugs into nude, severe combined immunodeficient mice (NOD/SCID). Conclusion-The present study represents an important conceptual development, suggesting that SPCs may contribute to extracellular matrix production. (Arterioscler Thromb Vasc Biol. 2010; 30: 1325-1332.)",

author = "David Simper and Ursula Mayr and Carmen Urbich and Anna Zampetaki and Marianna Prokopi and Athanasios Didangelos and Angelika Saje and Michael Mueller and Ulrike Benbow and Newby, {Andrew C.} and Rolf Apweiler and Salman Rahman and Stefanie Dimmeler and Qingbo Xu and Manuel Mayr",

year = "2010",

month = jul,

doi = "10.1161/ATVBAHA.110.204651",

language = "English",

volume = "30",

pages = "1325 -- U127",

journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",

publisher = "Lippincott Williams and Wilkins",

number = "7",

}

Simper, D, Mayr, U, Urbich, C, Zampetaki, A, Prokopi, M, Didangelos, A , Saje, A, Mueller, M, Benbow, U, Newby, AC, Apweiler, R, Rahman, S, Dimmeler, S, Xu, Q & Mayr, M 2010, 'Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 30, no. 7, pp. 1325 - U127. https://doi.org/10.1161/ATVBAHA.110.204651

TY - JOUR

T1 - Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production

AU - Simper, David

AU - Mayr, Ursula

AU - Urbich, Carmen

AU - Zampetaki, Anna

AU - Prokopi, Marianna

AU - Didangelos, Athanasios

AU - Saje, Angelika

AU - Mueller, Michael

AU - Benbow, Ulrike

AU - Newby, Andrew C.

AU - Apweiler, Rolf

AU - Rahman, Salman

AU - Dimmeler, Stefanie

AU - Xu, Qingbo

AU - Mayr, Manuel

PY - 2010/7

Y1 - 2010/7

N2 - Objective-Recent studies on cardiovascular progenitors have led to a new appreciation that paracrine factors may support the regeneration of damaged tissues. Methods and Results-We used a shotgun proteomics strategy to compare the secretome of peripheral blood-derived smooth muscle progenitors (SPCs) with human aortic smooth muscle cells. The late-outgrowth SPCs produced fewer proteolytic enzymes and inflammatory cytokines and showed reduced invasive capacity. Similar to smooth muscle cells, SPCs secreted extracellular matrix. However, SPCs produced different matrix proteins, as evidenced by the truncation of proangiogenic domains in collagen alpha-1 (I) and increased production of periostin. Moreover, SPCs retained serum proteins, including proteoglycans, regulating collagen assembly; and pigment epithelium-derived factor, a potent inhibitor of angiogenesis. As a functional consequence, their conditioned medium was less angiogenic, as demonstrated by endothelial tube formation assays in vitro and implantation of Matrigel plugs into nude, severe combined immunodeficient mice (NOD/SCID). Conclusion-The present study represents an important conceptual development, suggesting that SPCs may contribute to extracellular matrix production. (Arterioscler Thromb Vasc Biol. 2010; 30: 1325-1332.)

AB - Objective-Recent studies on cardiovascular progenitors have led to a new appreciation that paracrine factors may support the regeneration of damaged tissues. Methods and Results-We used a shotgun proteomics strategy to compare the secretome of peripheral blood-derived smooth muscle progenitors (SPCs) with human aortic smooth muscle cells. The late-outgrowth SPCs produced fewer proteolytic enzymes and inflammatory cytokines and showed reduced invasive capacity. Similar to smooth muscle cells, SPCs secreted extracellular matrix. However, SPCs produced different matrix proteins, as evidenced by the truncation of proangiogenic domains in collagen alpha-1 (I) and increased production of periostin. Moreover, SPCs retained serum proteins, including proteoglycans, regulating collagen assembly; and pigment epithelium-derived factor, a potent inhibitor of angiogenesis. As a functional consequence, their conditioned medium was less angiogenic, as demonstrated by endothelial tube formation assays in vitro and implantation of Matrigel plugs into nude, severe combined immunodeficient mice (NOD/SCID). Conclusion-The present study represents an important conceptual development, suggesting that SPCs may contribute to extracellular matrix production. (Arterioscler Thromb Vasc Biol. 2010; 30: 1325-1332.)

U2 - 10.1161/ATVBAHA.110.204651

DO - 10.1161/ATVBAHA.110.204651

M3 - Article

VL - 30

SP - 1325 - U127

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

IS - 7

ER -

Comparative Proteomics Profiling Reveals Role of Smooth Muscle Progenitors in Extracellular Matrix Production

Abstract

Access to Document

Fingerprint

Cite this