Comparative proteomics reveals recruitment patterns of some protein families in the venoms of Cnidaria

TY - JOUR

T1 - Comparative proteomics reveals recruitment patterns of some protein families in the venoms of Cnidaria

AU - Jaimes-Becerra, Adrian

AU - Chung, Ray

AU - Morandini, André C.

AU - Weston, Andrew

AU - Padilla, Gabriel

AU - Gacesa, Ranko

AU - Ward, Malcolm

AU - Long, Paul F.

AU - Marques, Antonio C.

PY - 2017/7/13

Y1 - 2017/7/13

N2 - Cnidarians are probably the oldest group of animals to be venomous, yet our current picture of cnidarian venom evolution is highly imbalanced due to limited taxon sampling. High-throughput tandem mass spectrometry was used to determine venom composition of the scyphozoan Chrysaora lactea and two cubozoans Tamoya haplonema and Chiropsalmus quadrumanus. Protein recruitment patterns were then compared against 5 other cnidarian venom proteomes taken from the literature. A total of 28 putative toxin protein families were identified, many for the first time in Cnidaria. Character mapping analysis revealed that 17 toxin protein families with predominantly cytolytic biological activities were likely recruited into the cnidarian venom proteome before the lineage split between Anthozoa and Medusozoa. Thereafter, venoms of Medusozoa and Anthozoa differed during subsequent divergence of cnidarian classes. Recruitment and loss of toxin protein families did not correlate with accepted phylogenetic patterns of Cnidaria. Selective pressures that drive toxin diversification independent of taxonomic positioning have yet to be identified in Cnidaria and now warrant experimental consideration.

AB - Cnidarians are probably the oldest group of animals to be venomous, yet our current picture of cnidarian venom evolution is highly imbalanced due to limited taxon sampling. High-throughput tandem mass spectrometry was used to determine venom composition of the scyphozoan Chrysaora lactea and two cubozoans Tamoya haplonema and Chiropsalmus quadrumanus. Protein recruitment patterns were then compared against 5 other cnidarian venom proteomes taken from the literature. A total of 28 putative toxin protein families were identified, many for the first time in Cnidaria. Character mapping analysis revealed that 17 toxin protein families with predominantly cytolytic biological activities were likely recruited into the cnidarian venom proteome before the lineage split between Anthozoa and Medusozoa. Thereafter, venoms of Medusozoa and Anthozoa differed during subsequent divergence of cnidarian classes. Recruitment and loss of toxin protein families did not correlate with accepted phylogenetic patterns of Cnidaria. Selective pressures that drive toxin diversification independent of taxonomic positioning have yet to be identified in Cnidaria and now warrant experimental consideration.

KW - Evolution

KW - Venom

KW - Cnidaria

KW - Nematocysts

KW - Proteomics

U2 - 10.1016/j.toxicon.2017.07.012

DO - 10.1016/j.toxicon.2017.07.012

M3 - Article

SN - 0041-0101

VL - 137

SP - 19

EP - 26

JO - Toxicon

JF - Toxicon

ER -