Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection

Jose Alonso Solis Lemus; Besaiz Sánchez Sánchez; Stefania Marcotti; Mubarik Burki; Brian Stramer; Constantino Carlos Reyes-Aldasoro

doi:10.3390/JIMAGING6050036

Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection

Jose Alonso Solis Lemus, Besaiz Sánchez Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro

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Abstract

In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis.

Original language	English
Article number	36
Journal	Journal of Imaging
Volume	6
Issue number	5
DOIs	https://doi.org/10.3390/JIMAGING6050036
Publication status	Published - 20 May 2020

Keywords

Cell segmentation
Cell shape
Cell tracking
Contact analysis
Macrophages

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title = "Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection",

abstract = "In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis.",

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AU - Marcotti, Stefania

AU - Burki, Mubarik

AU - Stramer, Brian

AU - Reyes-Aldasoro, Constantino Carlos

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AB - In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis.

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KW - Cell shape

KW - Cell tracking

KW - Contact analysis

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Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection

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