Complement regulator CD46 temporally regulates cytokine production by conventional and unconventional T cells

John Cardone, Gaelle Le Friec, Pierre Vantourout, Andrew Roberts, Anja Fuchs, Ian Jackson, Tesha Suddason, Graham Lord, John P. Atkinson, Andrew Cope, Adrian Hayday, Claudia Kemper

Research output: Contribution to journalArticlepeer-review

226 Citations (Scopus)

Abstract

In this study we demonstrate a new form of immunoregulation: engagement on CD4(+) T cells of the complement regulator CD46 promoted the effector potential of T helper type 1 cells (T(H)1 cells), but as interleukin 2 (IL-2) accumulated, it switched cells toward a regulatory phenotype, attenuating IL-2 production via the transcriptional regulator ICER/CREM and upregulating IL-10 after interaction of the CD46 tail with the serine-threonine kinase SPAK. Activated CD4(+) T cells produced CD46 ligands, and blocking CD46 inhibited IL-10 production. Furthermore, CD4(+) T cells in rheumatoid arthritis failed to switch, consequently producing excessive interferon-gamma (IFN-gamma). Finally, gamma delta T cells, which rarely produce IL-10, expressed an alternative CD46 isoform and were unable to switch. Nonetheless, coengagement of T cell antigen receptor (TCR) gamma delta and CD46 suppressed effector cytokine production, establishing that CD46 uses distinct mechanisms to regulate different T cell subsets during an immune response.
Original languageEnglish
Pages (from-to)862 - 871
Number of pages10
JournalNature Immunology
Volume11
Issue number9
DOIs
Publication statusPublished - Sept 2010

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