Confocal laser scanning microscopy and area-scale analysis used to quantify enamel surface textural changes from citric acid demineralization and salivary remineralization in vitro

TY - JOUR

T1 - Confocal laser scanning microscopy and area-scale analysis used to quantify enamel surface textural changes from citric acid demineralization and salivary remineralization in vitro

AU - Austin, Rupert

AU - Giusca, C. L.

AU - Macaulay, G.

AU - Moazzez, R.

AU - Bartlett, D. W.

PY - 2016/2

Y1 - 2016/2

N2 - Objectives. This paper investigates the application of confocal laser scanning microscopy to determine the effect of acid-mediated erosive enamel wear on the micro-texture of polished human enamel in vitro.Methods. Twenty polished enamel samples were prepared and subjected to a citric acid erosion and pooled human saliva remineralization model. Enamel surface microhardness was measured using a Knoop hardness tester, which confirmed that an early enamel erosion lesion was formed which was then subsequently completely remineralized. A confocal laser scanning microscope was used to capture high-resolution images of the enamel surfaces undergoing demineralization and remineralization. Area-scale analysis was used to identify the optimal feature size following which the surface texture was determined using the 3D (areal) texture parameter Sa.Results. The Sa successfully characterized the enamel erosion and remineralization for the polished enamel samples (P <0.001).Significance. Areal surface texture characterization of the surface events occurring during enamel demineralization and remineralization requires optical imaging instrumentation with lateral resolution

AB - Objectives. This paper investigates the application of confocal laser scanning microscopy to determine the effect of acid-mediated erosive enamel wear on the micro-texture of polished human enamel in vitro.Methods. Twenty polished enamel samples were prepared and subjected to a citric acid erosion and pooled human saliva remineralization model. Enamel surface microhardness was measured using a Knoop hardness tester, which confirmed that an early enamel erosion lesion was formed which was then subsequently completely remineralized. A confocal laser scanning microscope was used to capture high-resolution images of the enamel surfaces undergoing demineralization and remineralization. Area-scale analysis was used to identify the optimal feature size following which the surface texture was determined using the 3D (areal) texture parameter Sa.Results. The Sa successfully characterized the enamel erosion and remineralization for the polished enamel samples (P <0.001).Significance. Areal surface texture characterization of the surface events occurring during enamel demineralization and remineralization requires optical imaging instrumentation with lateral resolution

KW - Erosion

KW - Surface texture

KW - Remineralization

KW - Enamel Imaging

KW - Microhardness

KW - DENTAL EROSION

KW - PROFILOMETRY

KW - PELLICLE

KW - VIVO

KW - WEAR

UR - http://www.sciencedirect.com/science/article/pii/S0109564115004741

U2 - 10.1016/j.dental.2015.11.016

DO - 10.1016/j.dental.2015.11.016

M3 - Article

SN - 0109-5641

VL - 32

SP - 278

EP - 284

JO - Dental Materials

JF - Dental Materials

IS - 2

ER -