Conjugated linoleic acid isomers exert differential effects on an adipocyte model of HIV-associated lipodystrophy

Cathríona R. Loonam*, Sandra D. O’Dell, Paul Sharp, Anne Mullen

*Corresponding author for this work

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Abstract

Background: HIV-associated lipodystrophy is associated with decreased expression of PPAR-γ in adipose tissue. Conjugated linoleic acid (CLA) isomers (cis9, trans11 and trans10, cis12) are putative PPAR-γ agonists, but have not previously been investigated in the context of HIVassociated lipodystrophy. Method: 3T3-L1 pre-adipocytes were differentiated in the presence of ritonavir (20 μM as per previous experimental models) and 100 μM cis9,trans11, trans10,cis12 or vehicle control, DMSO. Microarray analysis, RT-PCR, DNA binding ELISA and Oil Red O staining were used to investigate adipocyte gene expression and binding, protein secretion and triglyceride storage. Results: trans10, cis12 + ritonavir altered the expression of 2160 gene transcripts greater than 1.5-fold compared with control, while 257 gene transcripts were altered by cis9,trans11 + ritonavir (P

Original languageEnglish
Pages (from-to)46-55
Number of pages10
JournalCURRENT HIV RESEARCH
Volume15
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Antiretroviral therapy
  • Conjugated linoleic acid
  • HIV-associated lipodystrophy
  • PPAR-γ
  • Ritonavir

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