Conjugated linoleic acid isomers exert differential effects on an adipocyte model of HIV-associated lipodystrophy

Cathríona R. Loonam; Sandra D. O’Dell; Paul Sharp; Anne Mullen

doi:10.2174/1570162X14666161207125948

Conjugated linoleic acid isomers exert differential effects on an adipocyte model of HIV-associated lipodystrophy

Cathríona R. Loonam^*, Sandra D. O’Dell, Paul Sharp, Anne Mullen

^*Corresponding author for this work

King's College London

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: HIV-associated lipodystrophy is associated with decreased expression of PPAR-γ in adipose tissue. Conjugated linoleic acid (CLA) isomers (cis9, trans11 and trans10, cis12) are putative PPAR-γ agonists, but have not previously been investigated in the context of HIVassociated lipodystrophy. Method: 3T3-L1 pre-adipocytes were differentiated in the presence of ritonavir (20 μM as per previous experimental models) and 100 μM cis9,trans11, trans10,cis12 or vehicle control, DMSO. Microarray analysis, RT-PCR, DNA binding ELISA and Oil Red O staining were used to investigate adipocyte gene expression and binding, protein secretion and triglyceride storage. Results: trans10, cis12 + ritonavir altered the expression of 2160 gene transcripts greater than 1.5-fold compared with control, while 257 gene transcripts were altered by cis9,trans11 + ritonavir (P

Original language	English
Pages (from-to)	46-55
Number of pages	10
Journal	CURRENT HIV RESEARCH
Volume	15
Issue number	1
DOIs	https://doi.org/10.2174/1570162X14666161207125948
Publication status	Published - 1 Jan 2017

Keywords

Antiretroviral therapy
Conjugated linoleic acid
HIV-associated lipodystrophy
PPAR-γ
Ritonavir

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2174/1570162X14666161207125948

Conjugated linoleic acid isomers_LOONAM_Published1January2017_GREEN AAMAccepted author manuscript, 922 KB

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title = "Conjugated linoleic acid isomers exert differential effects on an adipocyte model of HIV-associated lipodystrophy",

abstract = "Background: HIV-associated lipodystrophy is associated with decreased expression of PPAR-γ in adipose tissue. Conjugated linoleic acid (CLA) isomers (cis9, trans11 and trans10, cis12) are putative PPAR-γ agonists, but have not previously been investigated in the context of HIVassociated lipodystrophy. Method: 3T3-L1 pre-adipocytes were differentiated in the presence of ritonavir (20 μM as per previous experimental models) and 100 μM cis9,trans11, trans10,cis12 or vehicle control, DMSO. Microarray analysis, RT-PCR, DNA binding ELISA and Oil Red O staining were used to investigate adipocyte gene expression and binding, protein secretion and triglyceride storage. Results: trans10, cis12 + ritonavir altered the expression of 2160 gene transcripts greater than 1.5-fold compared with control, while 257 gene transcripts were altered by cis9,trans11 + ritonavir (P",

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AU - Loonam, Cathríona R.

AU - O’Dell, Sandra D.

AU - Sharp, Paul

AU - Mullen, Anne

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: HIV-associated lipodystrophy is associated with decreased expression of PPAR-γ in adipose tissue. Conjugated linoleic acid (CLA) isomers (cis9, trans11 and trans10, cis12) are putative PPAR-γ agonists, but have not previously been investigated in the context of HIVassociated lipodystrophy. Method: 3T3-L1 pre-adipocytes were differentiated in the presence of ritonavir (20 μM as per previous experimental models) and 100 μM cis9,trans11, trans10,cis12 or vehicle control, DMSO. Microarray analysis, RT-PCR, DNA binding ELISA and Oil Red O staining were used to investigate adipocyte gene expression and binding, protein secretion and triglyceride storage. Results: trans10, cis12 + ritonavir altered the expression of 2160 gene transcripts greater than 1.5-fold compared with control, while 257 gene transcripts were altered by cis9,trans11 + ritonavir (P

AB - Background: HIV-associated lipodystrophy is associated with decreased expression of PPAR-γ in adipose tissue. Conjugated linoleic acid (CLA) isomers (cis9, trans11 and trans10, cis12) are putative PPAR-γ agonists, but have not previously been investigated in the context of HIVassociated lipodystrophy. Method: 3T3-L1 pre-adipocytes were differentiated in the presence of ritonavir (20 μM as per previous experimental models) and 100 μM cis9,trans11, trans10,cis12 or vehicle control, DMSO. Microarray analysis, RT-PCR, DNA binding ELISA and Oil Red O staining were used to investigate adipocyte gene expression and binding, protein secretion and triglyceride storage. Results: trans10, cis12 + ritonavir altered the expression of 2160 gene transcripts greater than 1.5-fold compared with control, while 257 gene transcripts were altered by cis9,trans11 + ritonavir (P

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KW - Conjugated linoleic acid

KW - HIV-associated lipodystrophy

KW - PPAR-γ

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Conjugated linoleic acid isomers exert differential effects on an adipocyte model of HIV-associated lipodystrophy

Abstract

Keywords

UN SDGs

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