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CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics

Research output: Working paperPre-print

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CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics. / Danovi, Davide.

2020.

Research output: Working paperPre-print

Harvard

Danovi, D 2020 'CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics'. https://doi.org/https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1

APA

Danovi, D. (2020). CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics. https://doi.org/https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1

Vancouver

Danovi D. CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics. 2020 May 20. https://doi.org/https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1

Author

Danovi, Davide. / CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics. 2020.

Bibtex Download

@techreport{835cff53967140e1bdf71023ff139a6b,
title = "CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics",
abstract = "High throughput imaging methods can be applied to relevant cell culture models, fostering their use in research and translational applications. Improvements in microscopy, computational capabilities and data analysis have enabled high-throughput, high-content approaches from endpoint 2D microscopy images. Nonetheless, trade-offs in acquisition, computation and storage between content and throughput remain, in particular when cells and cell structures are imaged in 3D. Moreover, live 3D phase contrast microscopy images are not often amenable to analysis because of the high level of background noise.Cultures of Human induced pluripotent stem cells (hiPSC) offer unprecedented scope to profile and screen conditions affecting cell fate decisions, self-organisation and early embryonic development. However, quantifying changes in the morphology or function of cell structures derived from hiPSCs over time presents significant challenges. Here, we report a novel method based on the analysis of live phase contrast microscopy images of hiPSC spheroids. We compare self-renewing versus differentiating media conditions, which give rise to spheroids with distinct morphologies; round versus branched, respectively. These cell structures are segmented from 2D projections and analysed based on frame-to-frame variations. Importantly, a tailored convolutional neural network is trained and applied to predict culture conditions from time-frame images.We compare our results with more classic and involved endpoint 3D confocal microscopy and propose that such approaches can complement spheroid-based assays developed for the purpose of screening and profiling. This workflow can be realistically implemented in laboratories using imaging-based high-throughput methods for regenerative medicine and drug discovery.",
author = "Davide Danovi",
year = "2020",
month = "5",
day = "20",
doi = "https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1",
language = "English",
type = "WorkingPaper",

}

RIS (suitable for import to EndNote) Download

TY - UNPB

T1 - CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics

AU - Danovi, Davide

PY - 2020/5/20

Y1 - 2020/5/20

N2 - High throughput imaging methods can be applied to relevant cell culture models, fostering their use in research and translational applications. Improvements in microscopy, computational capabilities and data analysis have enabled high-throughput, high-content approaches from endpoint 2D microscopy images. Nonetheless, trade-offs in acquisition, computation and storage between content and throughput remain, in particular when cells and cell structures are imaged in 3D. Moreover, live 3D phase contrast microscopy images are not often amenable to analysis because of the high level of background noise.Cultures of Human induced pluripotent stem cells (hiPSC) offer unprecedented scope to profile and screen conditions affecting cell fate decisions, self-organisation and early embryonic development. However, quantifying changes in the morphology or function of cell structures derived from hiPSCs over time presents significant challenges. Here, we report a novel method based on the analysis of live phase contrast microscopy images of hiPSC spheroids. We compare self-renewing versus differentiating media conditions, which give rise to spheroids with distinct morphologies; round versus branched, respectively. These cell structures are segmented from 2D projections and analysed based on frame-to-frame variations. Importantly, a tailored convolutional neural network is trained and applied to predict culture conditions from time-frame images.We compare our results with more classic and involved endpoint 3D confocal microscopy and propose that such approaches can complement spheroid-based assays developed for the purpose of screening and profiling. This workflow can be realistically implemented in laboratories using imaging-based high-throughput methods for regenerative medicine and drug discovery.

AB - High throughput imaging methods can be applied to relevant cell culture models, fostering their use in research and translational applications. Improvements in microscopy, computational capabilities and data analysis have enabled high-throughput, high-content approaches from endpoint 2D microscopy images. Nonetheless, trade-offs in acquisition, computation and storage between content and throughput remain, in particular when cells and cell structures are imaged in 3D. Moreover, live 3D phase contrast microscopy images are not often amenable to analysis because of the high level of background noise.Cultures of Human induced pluripotent stem cells (hiPSC) offer unprecedented scope to profile and screen conditions affecting cell fate decisions, self-organisation and early embryonic development. However, quantifying changes in the morphology or function of cell structures derived from hiPSCs over time presents significant challenges. Here, we report a novel method based on the analysis of live phase contrast microscopy images of hiPSC spheroids. We compare self-renewing versus differentiating media conditions, which give rise to spheroids with distinct morphologies; round versus branched, respectively. These cell structures are segmented from 2D projections and analysed based on frame-to-frame variations. Importantly, a tailored convolutional neural network is trained and applied to predict culture conditions from time-frame images.We compare our results with more classic and involved endpoint 3D confocal microscopy and propose that such approaches can complement spheroid-based assays developed for the purpose of screening and profiling. This workflow can be realistically implemented in laboratories using imaging-based high-throughput methods for regenerative medicine and drug discovery.

U2 - https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1

DO - https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1

M3 - Pre-print

BT - CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics

ER -

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