Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

TY - JOUR

T1 - Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

AU - Płoskoń, Eliza

AU - Wagner, Sara C

AU - Ellington, Andrew D

AU - Jewett, Michael C

AU - O'Reilly, Rachel

AU - Booth, Paula J

PY - 2015

Y1 - 2015

N2 - DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.

AB - DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.

U2 - 10.1021/bc500473s

DO - 10.1021/bc500473s

M3 - Article

C2 - 25603321

SN - 1043-1802

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

ER -