TY - JOUR
T1 - Controlled Release of Human Dental Pulp Stem Cell-Derived Exosomes from Hydrogels Attenuates Temporomandibular Joint Osteoarthritis
AU - Diez-Guardia, Victor
AU - Tian, Yajing
AU - Guo, Yunzhe
AU - Li, Jiaying
AU - Cui, Shengjie
AU - Dreiss, Cécile A.
AU - Gentleman, Eileen
AU - Wang, Xuedong
N1 - Publisher Copyright:
© 2024 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH.
PY - 2024
Y1 - 2024
N2 - Temporomandibular joint osteoarthritis (TMJOA) is a painful inflammatory condition that limits mouth opening. Cell-derived exosomes, which have anti-inflammatory effects, are emerging as a treatment for TMJOA. Injection of dental pulp stem cells (DPSCs), which secrete exosomes, can moderate tissue damage in a rat model of TMJOA. However, injected exosomes are quickly cleared, necessitating repeated injections for therapeutic efficacy. Here, vinyl sulfone-modified hyaluronic acid (HA-VS) hydrogels, suitable for encapsulating exosomes are formulated. HA-VS hydrogels degrade in the presence of hyaluronidase and allow for the release of beads of similar size to exosomes over 3 to 6 days. In a rat model of TMJOA, injection of exosomes or exosomes within HA-VS hydrogels significantly attenuated damage-mediated subchondral bone loss as determined by micro-computed tomography, and reduced inflammatory and tissue damage scores as assessed by histology. Overall, DPSCs-derived exosomes attenuated joint damage, but treatment with exosomes within HA-VS hydrogels shows additional protective effects on subchondral bone maintenance and integrity. These findings confirm the protective effects of DPSCs-derived exosomes in moderating tissue damage in TMJOA and suggest that combining exosomes with HA hydrogels can further promote their therapeutic effects.
AB - Temporomandibular joint osteoarthritis (TMJOA) is a painful inflammatory condition that limits mouth opening. Cell-derived exosomes, which have anti-inflammatory effects, are emerging as a treatment for TMJOA. Injection of dental pulp stem cells (DPSCs), which secrete exosomes, can moderate tissue damage in a rat model of TMJOA. However, injected exosomes are quickly cleared, necessitating repeated injections for therapeutic efficacy. Here, vinyl sulfone-modified hyaluronic acid (HA-VS) hydrogels, suitable for encapsulating exosomes are formulated. HA-VS hydrogels degrade in the presence of hyaluronidase and allow for the release of beads of similar size to exosomes over 3 to 6 days. In a rat model of TMJOA, injection of exosomes or exosomes within HA-VS hydrogels significantly attenuated damage-mediated subchondral bone loss as determined by micro-computed tomography, and reduced inflammatory and tissue damage scores as assessed by histology. Overall, DPSCs-derived exosomes attenuated joint damage, but treatment with exosomes within HA-VS hydrogels shows additional protective effects on subchondral bone maintenance and integrity. These findings confirm the protective effects of DPSCs-derived exosomes in moderating tissue damage in TMJOA and suggest that combining exosomes with HA hydrogels can further promote their therapeutic effects.
KW - dental pulp stem cells
KW - exosomes
KW - hydrogel
KW - osteoarthritis
KW - temporomandibular joint
KW - tissue repair
UR - http://www.scopus.com/inward/record.url?scp=85212772999&partnerID=8YFLogxK
U2 - 10.1002/adhm.202402923
DO - 10.1002/adhm.202402923
M3 - Article
AN - SCOPUS:85212772999
SN - 2192-2640
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
ER -