Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube

TY - JOUR

T1 - Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube

AU - Symonds, Andrew C.

AU - Buckley, Clare E.

AU - Williams, Charlotte A.

AU - Clarke, Jonathan D.W.

PY - 2020/12/23

Y1 - 2020/12/23

N2 - Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells.

AB - Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells.

KW - Adhesions

KW - Epithelial tube

KW - Morphogenesis

KW - Neural tube

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85099075922&partnerID=8YFLogxK

U2 - 10.1242/dev.191494

DO - 10.1242/dev.191494

M3 - Article

C2 - 33361092

AN - SCOPUS:85099075922

SN - 0950-1991

VL - 147

JO - Development (Cambridge, England)

JF - Development (Cambridge, England)

IS - 24

ER -