Correction of time-resolved SPAD array measurements for accurate single-photon time-resolved biological imaging

Jakub Nedbal*, Francescopaolo Mattioli Della Rocca, Richard J. Walker, Robert K. Henderson, Klaus Suhling

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paper

7 Citations (Scopus)

Abstract

A 128x192 SPAD array (QuantiCam) with an on-chip time-to-digital converter in each pixel is used as a camera in a single-photon time-resolved fluorescence microscope. The SPAD array introduces systematic nonlinearities and timing offset to the measured photon arrival times. This limits the fidelity of the experimental results. A Monte-Carlo algorithm was developed to transform the raw photon time-stamp stream coming from the SPAD array into a corrected virtual “photon” time-stamp stream devoid of the systematic measurement errors. This data is compatible with existing downstream data processing pipelines used in time-correlated single-photon counting. We discuss the calibration measurement, the algorithm, their performance and application to live fluorescence lifetime imaging of photosynthetic organisms.
Original languageEnglish
Title of host publicationAdvanced Photon Counting Techniques XV
EditorsMark A. Itzler, Joshua C. Bienfang, K. Alex McIntosh
PublisherSPIE
Number of pages14
Volume11721
ISBN (Electronic)9781510642799
DOIs
Publication statusPublished - 12 Apr 2021

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11721
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

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