Cortical somatostatin long-range projection neurons and interneurons exhibit divergent developmental trajectories

Josephine Fisher, Marieke Verhagen, Zhen Long, Monika Moissidis, Yiming Yan, Chenyi He, Jingyu Wang, Elia Micoli, Clara Milían Alastruey, Rani Moors, Oscar Marín, Da Mi, Lynette Lim

Research output: Contribution to journalArticlepeer-review


The mammalian cerebral cortex contains an extraordinary diversity of cell types that emerge by implementing different developmental programs. Delineating when and how cellular diversification occurs is particularly challenging for cortical inhibitory neurons because they represent a small proportion of all cortical cells and have a protracted development. Here, we combine single-cell RNA sequencing and spatial transcriptomics to characterize the emergence of neuronal diversity among somatostatin-expressing (SST+) cells in mice. We found that SST+ inhibitory neurons segregate during embryonic stages into long-range projection (LRP) neurons and two types of interneurons, Martinotti cells and non-Martinotti cells, following distinct developmental trajectories. Two main subtypes of LRP neurons and several subtypes of interneurons are readily distinguishable in the embryo, although interneuron diversity is likely refined during early postnatal life. Our results suggest that the timing for cellular diversification is unique for different subtypes of SST+ neurons and particularly divergent for LRP neurons and interneurons.
Original languageEnglish
Pages (from-to)558-573.e8
Issue number4
Early online date11 Nov 2023
Publication statusPublished - 21 Feb 2024


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