Cranial suture lineage and contributions to repair of the mouse skull

Daniel Doro Pereira; Annie Liu; Jia Shang Lau; Arun Kumar Rajendra; Chris Healy; Marko Krstic; Agamemnon Grigoriadis; Sachiko Iseki; Karen Liu

Cranial suture lineage and contributions to repair of the mouse skull

Daniel Doro Pereira, Annie Liu, Jia Shang Lau, Arun Kumar Rajendra, Chris Healy, Marko Krstic, Agamemnon Grigoriadis, Sachiko Iseki, Karen Liu

Centre for Craniofacial & Regenerative Biology

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Abstract

The cranial sutures are proposed to be a stem cell niche, harbouring skeletal stem cells (SSCs) that are directly involved in development, homeostasis, and healing. Like the craniofacial bones, the sutures are formed from both mesoderm and neural crest. During cranial bone repair, neural crest cells have been proposed to be key players; however, neural crest contributions to adult sutures are not well defined, and the relative importance of suture proximity is unclear. Here, we use genetic approaches to re-examine the neural crest-mesoderm boundaries in the adult mouse skull. These are combined with calvarial wounding experiments suggesting that suture proximity improves the efficiency of cranial repair. Furthermore, we demonstrate that Gli1+ and Axin2+ SSCs are present in all calvarial sutures examined. We propose that the position of the defect determines the availability of neural crest-derived progenitors, which appear to be a key element in the repair of calvarial defects.

Original language	English
Journal	Development
Publication status	Published - 12 Feb 2024

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Doroetal2024-acceptedAccepted author manuscript, 14.8 MBLicence: CC BY

https://doi.org/10.1242/dev.202116Licence: CC BY

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title = "Cranial suture lineage and contributions to repair of the mouse skull",

abstract = "The cranial sutures are proposed to be a stem cell niche, harbouring skeletal stem cells (SSCs) that are directly involved in development, homeostasis, and healing. Like the craniofacial bones, the sutures are formed from both mesoderm and neural crest. During cranial bone repair, neural crest cells have been proposed to be key players; however, neural crest contributions to adult sutures are not well defined, and the relative importance of suture proximity is unclear. Here, we use genetic approaches to re-examine the neural crest-mesoderm boundaries in the adult mouse skull. These are combined with calvarial wounding experiments suggesting that suture proximity improves the efficiency of cranial repair. Furthermore, we demonstrate that Gli1+ and Axin2+ SSCs are present in all calvarial sutures examined. We propose that the position of the defect determines the availability of neural crest-derived progenitors, which appear to be a key element in the repair of calvarial defects.",

author = "{Doro Pereira}, Daniel and Annie Liu and Lau, {Jia Shang} and Rajendra, {Arun Kumar} and Chris Healy and Marko Krstic and Agamemnon Grigoriadis and Sachiko Iseki and Karen Liu",

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language = "English",

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T1 - Cranial suture lineage and contributions to repair of the mouse skull

AU - Doro Pereira, Daniel

AU - Liu, Annie

AU - Lau, Jia Shang

AU - Rajendra, Arun Kumar

AU - Healy, Chris

AU - Krstic, Marko

AU - Grigoriadis, Agamemnon

AU - Iseki, Sachiko

AU - Liu, Karen

PY - 2024/2/12

Y1 - 2024/2/12

N2 - The cranial sutures are proposed to be a stem cell niche, harbouring skeletal stem cells (SSCs) that are directly involved in development, homeostasis, and healing. Like the craniofacial bones, the sutures are formed from both mesoderm and neural crest. During cranial bone repair, neural crest cells have been proposed to be key players; however, neural crest contributions to adult sutures are not well defined, and the relative importance of suture proximity is unclear. Here, we use genetic approaches to re-examine the neural crest-mesoderm boundaries in the adult mouse skull. These are combined with calvarial wounding experiments suggesting that suture proximity improves the efficiency of cranial repair. Furthermore, we demonstrate that Gli1+ and Axin2+ SSCs are present in all calvarial sutures examined. We propose that the position of the defect determines the availability of neural crest-derived progenitors, which appear to be a key element in the repair of calvarial defects.

AB - The cranial sutures are proposed to be a stem cell niche, harbouring skeletal stem cells (SSCs) that are directly involved in development, homeostasis, and healing. Like the craniofacial bones, the sutures are formed from both mesoderm and neural crest. During cranial bone repair, neural crest cells have been proposed to be key players; however, neural crest contributions to adult sutures are not well defined, and the relative importance of suture proximity is unclear. Here, we use genetic approaches to re-examine the neural crest-mesoderm boundaries in the adult mouse skull. These are combined with calvarial wounding experiments suggesting that suture proximity improves the efficiency of cranial repair. Furthermore, we demonstrate that Gli1+ and Axin2+ SSCs are present in all calvarial sutures examined. We propose that the position of the defect determines the availability of neural crest-derived progenitors, which appear to be a key element in the repair of calvarial defects.

M3 - Article

SN - 0950-1991

JO - Development

JF - Development

ER -

Cranial suture lineage and contributions to repair of the mouse skull

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