Cross reactivity of polyclonal GFAP antiserum: implications for the in-vitro characterisation of brain endothelium

TY - JOUR

T1 - Cross reactivity of polyclonal GFAP antiserum: implications for the in-vitro characterisation of brain endothelium

AU - Dolman, D E M

AU - Lawrenson, J G

AU - Abbott, N J

PY - 2004/6/25

Y1 - 2004/6/25

N2 - Following a recent claim, based on glial acidic fibrillary protein (GFAP) expression, that brain-derived astrocytes in culture are in fact endothelial cells, we immuno-labelled primary cultures of rat brain astrocytes and endothelium with various GFAP antisera. Both cell types stained positively with a polyclonal antibody, although monoclonal antiserum labelled only astrocytes. We conclude that staining of endothelial cells with the polyclonal GFAP antiserum is due to cross reactivity with another protein. (C) 2004 Elsevier B.V All rights reserved.

AB - Following a recent claim, based on glial acidic fibrillary protein (GFAP) expression, that brain-derived astrocytes in culture are in fact endothelial cells, we immuno-labelled primary cultures of rat brain astrocytes and endothelium with various GFAP antisera. Both cell types stained positively with a polyclonal antibody, although monoclonal antiserum labelled only astrocytes. We conclude that staining of endothelial cells with the polyclonal GFAP antiserum is due to cross reactivity with another protein. (C) 2004 Elsevier B.V All rights reserved.

UR - http://www.scopus.com/inward/record.url?scp=2442547203&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2004.03.033

DO - 10.1016/j.brainres.2004.03.033

M3 - Article

SN - 1873-2747

VL - 1012

SP - 185

EP - 186

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -