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Crystallization and initial crystallographic analysis of AafA: The major adhesive subunit of the enteroaggregative Escherichia coli AAF/II pilus

Research output: Contribution to journalArticle

Yi Yang, James A. Garnett, Stephen Matthews

Original languageEnglish
Pages (from-to)454-456
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number4
DOIs
Publication statusPublished - 1 Apr 2011

King's Authors

Abstract

AafA is the major adhesive pilin subunit of the aggregative adherence fimbriae (AAF) from enteroaggregative Escherichia coli, which play an important role by attaching to the host cells during the initial phase of bacterial colonization and invasion. AafA has been crystallized at pH 3.4 and diffraction data have been collected to 2.1 Å resolution. Molecular replacement was unsuccessful and selenomethionine-substituted protein and heavy-atom derivatives are being prepared for phasing.

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