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Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide

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Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide. / Mesnage, R; Clair, E; Gress, S; Then, C; Székács, A; Séralini, G-E.

In: Journal of Applied Toxicology, Vol. 33, No. 7, 07.2013, p. 695-9.

Research output: Contribution to journalArticle

Harvard

Mesnage, R, Clair, E, Gress, S, Then, C, Székács, A & Séralini, G-E 2013, 'Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide', Journal of Applied Toxicology, vol. 33, no. 7, pp. 695-9. https://doi.org/10.1002/jat.2712

APA

Mesnage, R., Clair, E., Gress, S., Then, C., Székács, A., & Séralini, G-E. (2013). Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide. Journal of Applied Toxicology, 33(7), 695-9. https://doi.org/10.1002/jat.2712

Vancouver

Mesnage R, Clair E, Gress S, Then C, Székács A, Séralini G-E. Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide. Journal of Applied Toxicology. 2013 Jul;33(7):695-9. https://doi.org/10.1002/jat.2712

Author

Mesnage, R ; Clair, E ; Gress, S ; Then, C ; Székács, A ; Séralini, G-E. / Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide. In: Journal of Applied Toxicology. 2013 ; Vol. 33, No. 7. pp. 695-9.

Bibtex Download

@article{ed25569a28a8494c9b3518ba1ae41824,
title = "Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide",
abstract = "The study of combined effects of pesticides represents a challenge for toxicology. In the case of the new growing generation of genetically modified (GM) plants with stacked traits, glyphosate-based herbicides (like Roundup) residues are present in the Roundup-tolerant edible plants (especially corns) and mixed with modified Bt insecticidal toxins that are produced by the GM plants themselves. The potential side effects of these combined pesticides on human cells are investigated in this work. Here we have tested for the very first time Cry1Ab and Cry1Ac Bt toxins (10 ppb to 100 ppm) on the human embryonic kidney cell line 293, as well as their combined actions with Roundup, within 24 h, on three biomarkers of cell death: measurements of mitochondrial succinate dehydrogenase, adenylate kinase release by membrane alterations and caspase 3/7 inductions. Cry1Ab caused cell death from 100 ppm. For Cry1Ac, under such conditions, no effects were detected. The Roundup tested alone from 1 to 20 000 ppm is necrotic and apoptotic from 50 ppm, far below agricultural dilutions (50{\%} lethal concentration 57.5 ppm). The only measured significant combined effect was that Cry1Ab and Cry1Ac reduced caspases 3/7 activations induced by Roundup; this could delay the activation of apoptosis. There was the same tendency for the other markers. In these results, we argue that modified Bt toxins are not inert on nontarget human cells, and that they can present combined side-effects with other residues of pesticides specific to GM plants.",
keywords = "Adenylate Kinase, Apoptosis, Bacterial Proteins, Biomarkers, Caspase 3, Caspase 7, Diet, Drug Interactions, Endotoxins, Glycine, HEK293 Cells, Hemolysin Proteins, Herbicides, Humans, Insecticides, Mitochondria, Necrosis, Pesticide Residues, Succinate Dehydrogenase",
author = "R Mesnage and E Clair and S Gress and C Then and A Sz{\'e}k{\'a}cs and G-E S{\'e}ralini",
note = "Copyright {\circledC} 2012 John Wiley & Sons, Ltd.",
year = "2013",
month = "7",
doi = "10.1002/jat.2712",
language = "English",
volume = "33",
pages = "695--9",
journal = "Journal of Applied Toxicology",
issn = "0260-437X",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Cytotoxicity on human cells of Cry1Ab and Cry1Ac Bt insecticidal toxins alone or with a glyphosate-based herbicide

AU - Mesnage, R

AU - Clair, E

AU - Gress, S

AU - Then, C

AU - Székács, A

AU - Séralini, G-E

N1 - Copyright © 2012 John Wiley & Sons, Ltd.

PY - 2013/7

Y1 - 2013/7

N2 - The study of combined effects of pesticides represents a challenge for toxicology. In the case of the new growing generation of genetically modified (GM) plants with stacked traits, glyphosate-based herbicides (like Roundup) residues are present in the Roundup-tolerant edible plants (especially corns) and mixed with modified Bt insecticidal toxins that are produced by the GM plants themselves. The potential side effects of these combined pesticides on human cells are investigated in this work. Here we have tested for the very first time Cry1Ab and Cry1Ac Bt toxins (10 ppb to 100 ppm) on the human embryonic kidney cell line 293, as well as their combined actions with Roundup, within 24 h, on three biomarkers of cell death: measurements of mitochondrial succinate dehydrogenase, adenylate kinase release by membrane alterations and caspase 3/7 inductions. Cry1Ab caused cell death from 100 ppm. For Cry1Ac, under such conditions, no effects were detected. The Roundup tested alone from 1 to 20 000 ppm is necrotic and apoptotic from 50 ppm, far below agricultural dilutions (50% lethal concentration 57.5 ppm). The only measured significant combined effect was that Cry1Ab and Cry1Ac reduced caspases 3/7 activations induced by Roundup; this could delay the activation of apoptosis. There was the same tendency for the other markers. In these results, we argue that modified Bt toxins are not inert on nontarget human cells, and that they can present combined side-effects with other residues of pesticides specific to GM plants.

AB - The study of combined effects of pesticides represents a challenge for toxicology. In the case of the new growing generation of genetically modified (GM) plants with stacked traits, glyphosate-based herbicides (like Roundup) residues are present in the Roundup-tolerant edible plants (especially corns) and mixed with modified Bt insecticidal toxins that are produced by the GM plants themselves. The potential side effects of these combined pesticides on human cells are investigated in this work. Here we have tested for the very first time Cry1Ab and Cry1Ac Bt toxins (10 ppb to 100 ppm) on the human embryonic kidney cell line 293, as well as their combined actions with Roundup, within 24 h, on three biomarkers of cell death: measurements of mitochondrial succinate dehydrogenase, adenylate kinase release by membrane alterations and caspase 3/7 inductions. Cry1Ab caused cell death from 100 ppm. For Cry1Ac, under such conditions, no effects were detected. The Roundup tested alone from 1 to 20 000 ppm is necrotic and apoptotic from 50 ppm, far below agricultural dilutions (50% lethal concentration 57.5 ppm). The only measured significant combined effect was that Cry1Ab and Cry1Ac reduced caspases 3/7 activations induced by Roundup; this could delay the activation of apoptosis. There was the same tendency for the other markers. In these results, we argue that modified Bt toxins are not inert on nontarget human cells, and that they can present combined side-effects with other residues of pesticides specific to GM plants.

KW - Adenylate Kinase

KW - Apoptosis

KW - Bacterial Proteins

KW - Biomarkers

KW - Caspase 3

KW - Caspase 7

KW - Diet

KW - Drug Interactions

KW - Endotoxins

KW - Glycine

KW - HEK293 Cells

KW - Hemolysin Proteins

KW - Herbicides

KW - Humans

KW - Insecticides

KW - Mitochondria

KW - Necrosis

KW - Pesticide Residues

KW - Succinate Dehydrogenase

U2 - 10.1002/jat.2712

DO - 10.1002/jat.2712

M3 - Article

C2 - 22337346

VL - 33

SP - 695

EP - 699

JO - Journal of Applied Toxicology

JF - Journal of Applied Toxicology

SN - 0260-437X

IS - 7

ER -

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