Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro

TY - JOUR

T1 - Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro

AU - Wyman, S

AU - Simpson, R J

AU - McKie, A T

AU - Sharp, P A

PY - 2008/6/11

Y1 - 2008/6/11

N2 - MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that Fe-59 uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

AB - MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that Fe-59 uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

U2 - 10.1016/j.febslet.2008.05.010

DO - 10.1016/j.febslet.2008.05.010

M3 - Article

VL - 582

SP - 1901

EP - 1906

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -