Decoding functional hematopoietic progenitor cells in the adult human lung

Catharina Conrad; Melia Magnen; Jessica Tsui; Harrison Wismer; Mohammad Naser; Urmila Venkataramani; Bushra Samad; Simon J Cleary; Longhui Qiu; Jennifer J Tian; Marco De Giovanni; Nicole Mende; Emmanuelle Passegue; Elisa Laurenti; Alexis J Combes; Mark R Looney

doi:10.21203/rs.3.rs-3576483/v1

Decoding functional hematopoietic progenitor cells in the adult human lung

Catharina Conrad, Melia Magnen, Jessica Tsui, Harrison Wismer, Mohammad Naser, Urmila Venkataramani, Bushra Samad, Simon J Cleary, Longhui Qiu, Jennifer J Tian, Marco De Giovanni, Nicole Mende, Emmanuelle Passegue, Elisa Laurenti, Alexis J Combes, Mark R Looney

Institute of Pharmaceutical Science

Research output: Working paper/Preprint › Preprint

Abstract

The bone marrow is the main site of blood cell production in adults, however, rare pools of hematopoietic stem and progenitor cells with self-renewal and differentiation potential have been found in extramedullary organs. The lung is primarily known for its role in gas exchange but has recently been described as a site of blood production in mice. Here, we show that functional hematopoietic precursors reside in the extravascular spaces of the human lung, at a frequency similar to the bone marrow, and are capable of proliferation and engraftment. The organ-specific gene signature of pulmonary and medullary CD34+ hematopoietic progenitors indicates greater baseline activation of immune, megakaryocyte/platelet and erythroid-related pathways in lung progenitors. Spatial transcriptomics mapped blood progenitors in the lung to a vascular-rich alveolar interstitium niche. These results identify the lung as a pool for uniquely programmed blood stem and progenitor cells with the potential to support hematopoiesis in humans.

Original language	English
DOIs	https://doi.org/10.21203/rs.3.rs-3576483/v1
Publication status	Published - 16 Feb 2024

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title = "Decoding functional hematopoietic progenitor cells in the adult human lung",

abstract = "The bone marrow is the main site of blood cell production in adults, however, rare pools of hematopoietic stem and progenitor cells with self-renewal and differentiation potential have been found in extramedullary organs. The lung is primarily known for its role in gas exchange but has recently been described as a site of blood production in mice. Here, we show that functional hematopoietic precursors reside in the extravascular spaces of the human lung, at a frequency similar to the bone marrow, and are capable of proliferation and engraftment. The organ-specific gene signature of pulmonary and medullary CD34+ hematopoietic progenitors indicates greater baseline activation of immune, megakaryocyte/platelet and erythroid-related pathways in lung progenitors. Spatial transcriptomics mapped blood progenitors in the lung to a vascular-rich alveolar interstitium niche. These results identify the lung as a pool for uniquely programmed blood stem and progenitor cells with the potential to support hematopoiesis in humans.",

author = "Catharina Conrad and Melia Magnen and Jessica Tsui and Harrison Wismer and Mohammad Naser and Urmila Venkataramani and Bushra Samad and Cleary, {Simon J} and Longhui Qiu and Tian, {Jennifer J} and {De Giovanni}, Marco and Nicole Mende and Emmanuelle Passegue and Elisa Laurenti and Combes, {Alexis J} and Looney, {Mark R}",

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T1 - Decoding functional hematopoietic progenitor cells in the adult human lung

AU - Conrad, Catharina

AU - Magnen, Melia

AU - Tsui, Jessica

AU - Wismer, Harrison

AU - Naser, Mohammad

AU - Venkataramani, Urmila

AU - Samad, Bushra

AU - Cleary, Simon J

AU - Qiu, Longhui

AU - Tian, Jennifer J

AU - De Giovanni, Marco

AU - Mende, Nicole

AU - Passegue, Emmanuelle

AU - Laurenti, Elisa

AU - Combes, Alexis J

AU - Looney, Mark R

PY - 2024/2/16

Y1 - 2024/2/16

N2 - The bone marrow is the main site of blood cell production in adults, however, rare pools of hematopoietic stem and progenitor cells with self-renewal and differentiation potential have been found in extramedullary organs. The lung is primarily known for its role in gas exchange but has recently been described as a site of blood production in mice. Here, we show that functional hematopoietic precursors reside in the extravascular spaces of the human lung, at a frequency similar to the bone marrow, and are capable of proliferation and engraftment. The organ-specific gene signature of pulmonary and medullary CD34+ hematopoietic progenitors indicates greater baseline activation of immune, megakaryocyte/platelet and erythroid-related pathways in lung progenitors. Spatial transcriptomics mapped blood progenitors in the lung to a vascular-rich alveolar interstitium niche. These results identify the lung as a pool for uniquely programmed blood stem and progenitor cells with the potential to support hematopoiesis in humans.

AB - The bone marrow is the main site of blood cell production in adults, however, rare pools of hematopoietic stem and progenitor cells with self-renewal and differentiation potential have been found in extramedullary organs. The lung is primarily known for its role in gas exchange but has recently been described as a site of blood production in mice. Here, we show that functional hematopoietic precursors reside in the extravascular spaces of the human lung, at a frequency similar to the bone marrow, and are capable of proliferation and engraftment. The organ-specific gene signature of pulmonary and medullary CD34+ hematopoietic progenitors indicates greater baseline activation of immune, megakaryocyte/platelet and erythroid-related pathways in lung progenitors. Spatial transcriptomics mapped blood progenitors in the lung to a vascular-rich alveolar interstitium niche. These results identify the lung as a pool for uniquely programmed blood stem and progenitor cells with the potential to support hematopoiesis in humans.

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DO - 10.21203/rs.3.rs-3576483/v1

M3 - Preprint

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BT - Decoding functional hematopoietic progenitor cells in the adult human lung

ER -

Decoding functional hematopoietic progenitor cells in the adult human lung

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