Defining APOBEC3 Expression Patterns in Human Tissues and Hematopoietic Cell Subsets

FA Koning, ENC Newman, EY Kim, KJ Kunstman, SM Wolinsky, MH Malim

Research output: Contribution to journalArticlepeer-review

271 Citations (Scopus)

Abstract

Human APOBEC3 enzymes are cellular DNA cytidine deaminases that inhibit and/or mutate a variety of retroviruses, retrotransposons, and DNA viruses. Here, we report a detailed examination of human APOBEC3 gene expression, focusing on APOBEC3G (A3G) and APOBEC3F (A3F), which are potent inhibitors of human immunodeficiency virus type 1 (HIV-1) infection but are suppressed by HIV-1 Vif.A3G and A3F are expressed widely in hematopoietic cell populations, including T cells, B cells, and myeloid cells, as well as in tissues where mRNA levels broadly correlate with the lymphoid cell content (gonadal tissues are exceptions). By measuring mRNA copy numbers, we find that A3G mRNA is similar to 10-fold more abundant than A3F mRNA, implying that A3G is the more significant anti-HIV-1 factor in vivo. A3G and A3F levels also vary between donors, and these differences are sustained over 12 months. Responses to T-cell activation or cytokines reveal that A3G and A3F mRNA levels are induced similar to 10-fold in macrophages and dendritic cells (DCs) by alpha interferon (IFN-alpha) and similar to 4-fold in naive CD4(+) T cells. However, immunoblotting revealed that A3G protein levels are induced by IFN-alpha in macrophages and DCs but not in T cells. In contrast, T-cell activation and IFN-gamma had a minimal impact on A3G or A3F expression. Finally, we noted that A3A mRNA expression and protein expression are exquisitely sensitive to IFN-alpha induction in CD4(+) T cells, macrophages, and DCs but not to T-cell activation or other cytokines. Given that A3A does not affect HIV-1 infection, these observations imply that this protein may participate in early antiviral innate immune responses.
Original languageEnglish
Pages (from-to)9474 - 9485
Number of pages12
JournalJournal of Virology
Volume83
Issue number18
DOIs
Publication statusPublished - Sept 2009

Fingerprint

Dive into the research topics of 'Defining APOBEC3 Expression Patterns in Human Tissues and Hematopoietic Cell Subsets'. Together they form a unique fingerprint.

Cite this