King's College London

Research portal

Defining the nociceptor transcriptome

Research output: Contribution to journalArticlepeer-review

Standard

Defining the nociceptor transcriptome. / Thakur, Matthew; Crow, Megan; Richards, Natalie et al.

In: Frontiers in Molecular Neuroscience, Vol. 7, 87, 11.11.2014.

Research output: Contribution to journalArticlepeer-review

Harvard

Thakur, M, Crow, M, Richards, N, Davey, GIJ, Levine, E, Kelleher, JH, Agley, CC, Denk, F, Harridge, SDR & McMahon, SB 2014, 'Defining the nociceptor transcriptome', Frontiers in Molecular Neuroscience, vol. 7, 87. https://doi.org/10.3389/fnmol.2014.00087

APA

Thakur, M., Crow, M., Richards, N., Davey, G. I. J., Levine, E., Kelleher, J. H., Agley, C. C., Denk, F., Harridge, S. D. R., & McMahon, S. B. (2014). Defining the nociceptor transcriptome. Frontiers in Molecular Neuroscience, 7, [87]. https://doi.org/10.3389/fnmol.2014.00087

Vancouver

Thakur M, Crow M, Richards N, Davey GIJ, Levine E, Kelleher JH et al. Defining the nociceptor transcriptome. Frontiers in Molecular Neuroscience. 2014 Nov 11;7. 87. https://doi.org/10.3389/fnmol.2014.00087

Author

Thakur, Matthew ; Crow, Megan ; Richards, Natalie et al. / Defining the nociceptor transcriptome. In: Frontiers in Molecular Neuroscience. 2014 ; Vol. 7.

Bibtex Download

@article{dbe67ad8536c44cdaa0abd3d2bd2b6d7,
title = "Defining the nociceptor transcriptome",
abstract = "Unbiased {"}omics{"} techniques, such as next generation RNA-sequencing, can provide entirely novel insights into biological systems. However, cellular heterogeneity presents a significant barrier to analysis and interpretation of these datasets. The neurons of the dorsal root ganglia (DRG) are an important model for studies of neuronal injury, regeneration and pain. The majority of investigators utilize a dissociated preparation of whole ganglia when studying cellular and molecular function. We demonstrate that the standard methods for producing these preparations gives a 10%-neuronal mixture of cells, with the remainder of cells constituting satellite glia and other non-neuronal cell types. Using a novel application of magnetic purification, we consistently obtain over 95% pure, viable neurons from adult tissue, significantly enriched for small diameter nociceptors expressing the voltage gated ion channel Nav1.8. Using genome-wide RNA-sequencing we compare the currently used (10% neuronal) and pure (95% nociceptor) preparations and find 920 genes enriched. This gives an unprecedented insight into the molecular composition of small nociceptive neurons in the DRG, potentially altering the interpretation of previous studies performed at the tissue level, and indicating a number of novel markers of this widely-studied population of cells. We anticipate that the ease of use, affordability and speed of this technique will see it become widely adopted, delivering a greatly improved capacity to study the roles of nociceptors in health and disease.",
author = "Matthew Thakur and Megan Crow and Natalie Richards and Davey, {Gareth I J} and Emma Levine and Kelleher, {Jayne H} and Agley, {Chibeza C} and Franziska Denk and Harridge, {Stephen D R} and McMahon, {Stephen B}",
year = "2014",
month = nov,
day = "11",
doi = "10.3389/fnmol.2014.00087",
language = "English",
volume = "7",
journal = "Frontiers in Molecular Neuroscience",
issn = "1662-5099",
publisher = "Frontiers Media",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Defining the nociceptor transcriptome

AU - Thakur, Matthew

AU - Crow, Megan

AU - Richards, Natalie

AU - Davey, Gareth I J

AU - Levine, Emma

AU - Kelleher, Jayne H

AU - Agley, Chibeza C

AU - Denk, Franziska

AU - Harridge, Stephen D R

AU - McMahon, Stephen B

PY - 2014/11/11

Y1 - 2014/11/11

N2 - Unbiased "omics" techniques, such as next generation RNA-sequencing, can provide entirely novel insights into biological systems. However, cellular heterogeneity presents a significant barrier to analysis and interpretation of these datasets. The neurons of the dorsal root ganglia (DRG) are an important model for studies of neuronal injury, regeneration and pain. The majority of investigators utilize a dissociated preparation of whole ganglia when studying cellular and molecular function. We demonstrate that the standard methods for producing these preparations gives a 10%-neuronal mixture of cells, with the remainder of cells constituting satellite glia and other non-neuronal cell types. Using a novel application of magnetic purification, we consistently obtain over 95% pure, viable neurons from adult tissue, significantly enriched for small diameter nociceptors expressing the voltage gated ion channel Nav1.8. Using genome-wide RNA-sequencing we compare the currently used (10% neuronal) and pure (95% nociceptor) preparations and find 920 genes enriched. This gives an unprecedented insight into the molecular composition of small nociceptive neurons in the DRG, potentially altering the interpretation of previous studies performed at the tissue level, and indicating a number of novel markers of this widely-studied population of cells. We anticipate that the ease of use, affordability and speed of this technique will see it become widely adopted, delivering a greatly improved capacity to study the roles of nociceptors in health and disease.

AB - Unbiased "omics" techniques, such as next generation RNA-sequencing, can provide entirely novel insights into biological systems. However, cellular heterogeneity presents a significant barrier to analysis and interpretation of these datasets. The neurons of the dorsal root ganglia (DRG) are an important model for studies of neuronal injury, regeneration and pain. The majority of investigators utilize a dissociated preparation of whole ganglia when studying cellular and molecular function. We demonstrate that the standard methods for producing these preparations gives a 10%-neuronal mixture of cells, with the remainder of cells constituting satellite glia and other non-neuronal cell types. Using a novel application of magnetic purification, we consistently obtain over 95% pure, viable neurons from adult tissue, significantly enriched for small diameter nociceptors expressing the voltage gated ion channel Nav1.8. Using genome-wide RNA-sequencing we compare the currently used (10% neuronal) and pure (95% nociceptor) preparations and find 920 genes enriched. This gives an unprecedented insight into the molecular composition of small nociceptive neurons in the DRG, potentially altering the interpretation of previous studies performed at the tissue level, and indicating a number of novel markers of this widely-studied population of cells. We anticipate that the ease of use, affordability and speed of this technique will see it become widely adopted, delivering a greatly improved capacity to study the roles of nociceptors in health and disease.

U2 - 10.3389/fnmol.2014.00087

DO - 10.3389/fnmol.2014.00087

M3 - Article

C2 - 25426020

VL - 7

JO - Frontiers in Molecular Neuroscience

JF - Frontiers in Molecular Neuroscience

SN - 1662-5099

M1 - 87

ER -

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454