Abstract
Objective To compare the outcome of transfer of thawed blastocysts frozen on either day 5 or day 6 after in vitro fertilisation.
Design Cohort observational study.
Setting Tertiary assisted conception unit in London, UK.
Population Six hundred and forty-two consecutive nondonor programmed thawed blastocyst transfer (TBT) cycles.
Methods High-grade blastocysts were frozen on day 5 (n = 314) or day 6 (n = 328) after fertilisation using a slow-freezing protocol. Endometrial preparation was performed using estradiol valerate. Progesterone supplementation was commenced when the endometrial thickness had reached 7 mm or more. Frozen blastocysts were thawed on day 6 of progesterone supplementation and assessed immediately after thawing for survival, and after 3–4 hours for blastocoele re-expansion.
Main outcome measures Thawed blastocyst survival and re-expansion rates, and pregnancy and live birth rates, per TBT.
Results Thawed blastocyst survival and re-expansion rates were comparable between the day 5 and day 6 groups (87% versus 87%, P = 0.50 and 73% versus 71%, P = 0.35, respectively). The live birth rate was similar between the two groups (29% versus 28.5%, P = 0.93, respectively). After adjusting for confounding variables, the odds ratio (OR) of a live birth in cycles in which the thawed blastocysts were frozen on day 6 compared with day 5 was 1.23 [95% confidence interval (CI), 0.81–1.86, P = 0.34].
Conclusion The pregnancy potential of high-grade blastocysts frozen on day 5 and day 6 after in vitro fertilisation and replaced in programmed TBT cycles is comparable.
Design Cohort observational study.
Setting Tertiary assisted conception unit in London, UK.
Population Six hundred and forty-two consecutive nondonor programmed thawed blastocyst transfer (TBT) cycles.
Methods High-grade blastocysts were frozen on day 5 (n = 314) or day 6 (n = 328) after fertilisation using a slow-freezing protocol. Endometrial preparation was performed using estradiol valerate. Progesterone supplementation was commenced when the endometrial thickness had reached 7 mm or more. Frozen blastocysts were thawed on day 6 of progesterone supplementation and assessed immediately after thawing for survival, and after 3–4 hours for blastocoele re-expansion.
Main outcome measures Thawed blastocyst survival and re-expansion rates, and pregnancy and live birth rates, per TBT.
Results Thawed blastocyst survival and re-expansion rates were comparable between the day 5 and day 6 groups (87% versus 87%, P = 0.50 and 73% versus 71%, P = 0.35, respectively). The live birth rate was similar between the two groups (29% versus 28.5%, P = 0.93, respectively). After adjusting for confounding variables, the odds ratio (OR) of a live birth in cycles in which the thawed blastocysts were frozen on day 6 compared with day 5 was 1.23 [95% confidence interval (CI), 0.81–1.86, P = 0.34].
Conclusion The pregnancy potential of high-grade blastocysts frozen on day 5 and day 6 after in vitro fertilisation and replaced in programmed TBT cycles is comparable.
| Original language | English |
|---|---|
| Pages (from-to) | 1551-1556 |
| Number of pages | 6 |
| Journal | Bjog-An international journal of obstetrics and gynaecology |
| Volume | 118 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - Dec 2011 |
Keywords
- Blastocyst
- blastocyst transfer
- embryo development
- embryo freezing
- endometrium
- in vitro fertilisation outcome
- IN-VITRO FERTILIZATION
- PREGNANCY RATES
- EMBRYO-TRANSFER
- ENDOMETRIAL RECEPTIVITY
- DAY-6 BLASTOCYSTS
- VITRIFICATION
- CRYOPRESERVATION
- IMPLANTATION
- REPLACEMENT
- CONCEPTION