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Objective: We have designed a low-cost, reusable incubation system that allows cells to be cultured in either plated or suspension culture under complete atmospheric control for radiotracer characterization. We demonstrate its utility here in the first quantification of the hypoxia-dependent accumulation of Cu-64-diacetyl bis(N4-methylthiosemicarbazone) (Cu-64-ATSM) in adult rat ventricular myocytes (ARVMs).
Materials and methods: ARVMs were allowed to adhere overnight in 9 cm culture plates (2x10(5) cells/dish) or were used in suspension culture, placed inside the chamber and equilibrated with either oxic (95 or 21% O-2/5% CO2) or anoxic gas (95% N-2/5% CO2). Cu-64-ATSM of 100 kBq was administered, and the cells were incubated for 30 or 60 min. Cells were then harvested, counted and fractionated to determine intracellular Cu-64 biodistribution.
Results: After 1 h, the average cellular Cu-64 retention in plated ARVMs under oxygenated conditions was 23.9 +/- 2.5 mBq/cell (95% O-2), increasing to 27.3 +/- 5.1 under 21% O-2 (P<0.05) and to 36.1 +/- 3.1 under 0% O-2 (P<0.05). When ARVMs were cultured in suspension, normoxic-hypoxic contrast was less marked but still significant [63.2 +/- 14.1 vs. 53.4 +/- 10.9% mBq/cell after 30 min (P<0.05)]. Sixty percent of tracer accumulated in the cytosol, and, although total cellular retention increased during hypoxia, there was no enrichment in any particular cellular compartment.
Conclusion: This apparatus allows the conduction of radiotracer uptake studies in cells under complete atmospheric control, as evidenced by our first demonstration of the hypoxia-dependent uptake of Cu-64-ATSM in ventricular myocytes. It is ideally suited for screening, validating and characterizing novel hypoxia-selective radiotracers.
- cardiac myocytes
- incubation chamber
- subcellular fractionation
- BIS(THIOSEMICARBAZONE) COMPLEXES
- COVERSLIP HYPOXIA
- IMAGING AGENTS