TY - JOUR
T1 - Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions
AU - Ilic, Dusko
AU - Stephenson, Emma
AU - Wood, Victoria
AU - Jacquet, Laureen
AU - Stevenson, Danielle
AU - Petrova, Anastasia
AU - Kadeva, Neli
AU - Codognotto, Stefano
AU - Patel, Heema
AU - Semple, Maxine
AU - Cornwell, Glenda
AU - Ogilvie, Caroline
AU - Braude, Peter
PY - 2012/1
Y1 - 2012/1
N2 - Background aims. Human embryonic stem (hES) cells hold great potential for cell therapy and regenerative medicine because of their pluripotency and capacity for self-renewal. The conditions used to derive and culture hES cells vary between and within laboratories depending on the desired use of the cells. Until recently, stem cell culture has been carried out using feeder cells, and culture media, that contain animal products. Recent advances in technology have opened up the possibility of both xeno-free and feeder-free culture of stem cells, essential conditions for the use of stem cells for clinical purposes. To date, however, there has been limited success in achieving this aim. Methods, results and conclusions. Protocols were developed for the successful derivation of two normal and three specific mutation-carrying (SMC) (Huntington's disease and myotonic dystrophy 1) genomically stable hES cell lines, and their adaptation to feeder-free culture, all under xeno-free conditions.
AB - Background aims. Human embryonic stem (hES) cells hold great potential for cell therapy and regenerative medicine because of their pluripotency and capacity for self-renewal. The conditions used to derive and culture hES cells vary between and within laboratories depending on the desired use of the cells. Until recently, stem cell culture has been carried out using feeder cells, and culture media, that contain animal products. Recent advances in technology have opened up the possibility of both xeno-free and feeder-free culture of stem cells, essential conditions for the use of stem cells for clinical purposes. To date, however, there has been limited success in achieving this aim. Methods, results and conclusions. Protocols were developed for the successful derivation of two normal and three specific mutation-carrying (SMC) (Huntington's disease and myotonic dystrophy 1) genomically stable hES cell lines, and their adaptation to feeder-free culture, all under xeno-free conditions.
U2 - 10.3109/14653249.2011.623692
DO - 10.3109/14653249.2011.623692
M3 - Article
VL - 14
SP - 122
EP - 128
JO - CYTOTHERAPY
JF - CYTOTHERAPY
IS - 1
ER -
