Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions

Dusko Ilic, Emma Stephenson, Victoria Wood, Laureen Jacquet, Danielle Stevenson, Anastasia Petrova, Neli Kadeva, Stefano Codognotto, Heema Patel, Maxine Semple, Glenda Cornwell, Caroline Ogilvie, Peter Braude

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)


Background aims. Human embryonic stem (hES) cells hold great potential for cell therapy and regenerative medicine because of their pluripotency and capacity for self-renewal. The conditions used to derive and culture hES cells vary between and within laboratories depending on the desired use of the cells. Until recently, stem cell culture has been carried out using feeder cells, and culture media, that contain animal products. Recent advances in technology have opened up the possibility of both xeno-free and feeder-free culture of stem cells, essential conditions for the use of stem cells for clinical purposes. To date, however, there has been limited success in achieving this aim. Methods, results and conclusions. Protocols were developed for the successful derivation of two normal and three specific mutation-carrying (SMC) (Huntington's disease and myotonic dystrophy 1) genomically stable hES cell lines, and their adaptation to feeder-free culture, all under xeno-free conditions.
Original languageEnglish
Pages (from-to)122 - 128
Number of pages7
Issue number1
Publication statusPublished - Jan 2012


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