Desflurane induces only minor Ca2+ release from the sarcoplasmic reticulum of mammalian skeletal muscle

G Kunst; A G Stucke; B M Graf; E Martin; R H Fink

doi:10.1097/00000542-200009000-00034

Desflurane induces only minor Ca2+ release from the sarcoplasmic reticulum of mammalian skeletal muscle

G Kunst, A G Stucke, B M Graf, E Martin, R H Fink

Randall Centre of Cell & Molecular Biophysics

Heidelberg University

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

BACKGROUND: Desflurane is a weaker trigger of malignant hyperthermia than is halothane. There are very few data of the pathophysiologic background of this observation. Therefore, the authors' aim was to investigate the direct effect of desflurane on calcium release in skinned skeletal muscle fibers.

METHODS: For the measurements, single saponin-skinned muscle fiber preparations of BALB/c mice were used. For Ca2+ release experiments, liquid desflurane at 0.6 and 3.5 mm was applied to weakly calcium-buffered solutions with no added Ca2+. Desflurane was diluted in strongly Ca2+-buffered solutions, with [Ca2+] between 3.0 and 24.9 micrometer for [Ca2+]-force relations. Force transients were transformed into Ca2+ transients based on the individual [Ca2+]-force relations. As controls, 30 mm caffeine and equimolar sevoflurane were investigated in the same muscle fibers.

RESULTS: At 3.5 mm, desflurane induced peak force transients of 8 +/- 4% (mean +/- SD) of maximal Ca2+-activated force (Tmax). These peak values were significantly smaller than those in the presence of 3.5 mm sevoflurane (24 +/- 10% of Tmax, P < 0.05), and 4 or 5 times smaller than previously reported Ca2+-release-induced force transients by equimolar halothane. Calculated peak Ca2+ transients derived from force transients and induced by 3.5 and 0.6 mm desflurane were significantly smaller than those induced by 30 mm caffeine. The [Ca2+]-force relation was shifted by desflurane, resulting in a Ca2+-sensitizing effect. The maximal Ca2+-activated force was significantly increased by 0.6 mm desflurane in comparison with the control, with no added substance (P </= 0.05).

CONCLUSION: Desflurane induces only slight Ca2+ release in skinned skeletal muscle fibers.

Original language	English
Pages (from-to)	832-6
Number of pages	5
Journal	Anesthesiology
Volume	93
Issue number	3
DOIs	https://doi.org/10.1097/00000542-200009000-00034
Publication status	Published - Sept 2000

Keywords

Anesthetics, Inhalation/pharmacology
Animals
Calcium/metabolism
Desflurane
Isoflurane/analogs & derivatives
Mice
Mice, Inbred BALB C
Muscle, Skeletal/drug effects
Sarcoplasmic Reticulum/drug effects

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10.1097/00000542-200009000-00034

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@article{ca02ae447bf2452e923ad7bdff8237dd,

title = "Desflurane induces only minor Ca2+ release from the sarcoplasmic reticulum of mammalian skeletal muscle",

abstract = "BACKGROUND: Desflurane is a weaker trigger of malignant hyperthermia than is halothane. There are very few data of the pathophysiologic background of this observation. Therefore, the authors' aim was to investigate the direct effect of desflurane on calcium release in skinned skeletal muscle fibers.METHODS: For the measurements, single saponin-skinned muscle fiber preparations of BALB/c mice were used. For Ca2+ release experiments, liquid desflurane at 0.6 and 3.5 mm was applied to weakly calcium-buffered solutions with no added Ca2+. Desflurane was diluted in strongly Ca2+-buffered solutions, with [Ca2+] between 3.0 and 24.9 micrometer for [Ca2+]-force relations. Force transients were transformed into Ca2+ transients based on the individual [Ca2+]-force relations. As controls, 30 mm caffeine and equimolar sevoflurane were investigated in the same muscle fibers.RESULTS: At 3.5 mm, desflurane induced peak force transients of 8 +/- 4% (mean +/- SD) of maximal Ca2+-activated force (Tmax). These peak values were significantly smaller than those in the presence of 3.5 mm sevoflurane (24 +/- 10% of Tmax, P < 0.05), and 4 or 5 times smaller than previously reported Ca2+-release-induced force transients by equimolar halothane. Calculated peak Ca2+ transients derived from force transients and induced by 3.5 and 0.6 mm desflurane were significantly smaller than those induced by 30 mm caffeine. The [Ca2+]-force relation was shifted by desflurane, resulting in a Ca2+-sensitizing effect. The maximal Ca2+-activated force was significantly increased by 0.6 mm desflurane in comparison with the control, with no added substance (P </= 0.05).CONCLUSION: Desflurane induces only slight Ca2+ release in skinned skeletal muscle fibers.",

keywords = "Anesthetics, Inhalation/pharmacology, Animals, Calcium/metabolism, Desflurane, Isoflurane/analogs & derivatives, Mice, Mice, Inbred BALB C, Muscle, Skeletal/drug effects, Sarcoplasmic Reticulum/drug effects",

author = "G Kunst and Stucke, {A G} and Graf, {B M} and E Martin and Fink, {R H}",

year = "2000",

month = sep,

doi = "10.1097/00000542-200009000-00034",

language = "English",

volume = "93",

pages = "832--6",

journal = "Anesthesiology",

issn = "0003-3022",

publisher = "Lippincott Williams and Wilkins",

number = "3",

}

TY - JOUR

T1 - Desflurane induces only minor Ca2+ release from the sarcoplasmic reticulum of mammalian skeletal muscle

AU - Kunst, G

AU - Stucke, A G

AU - Graf, B M

AU - Martin, E

AU - Fink, R H

PY - 2000/9

Y1 - 2000/9

N2 - BACKGROUND: Desflurane is a weaker trigger of malignant hyperthermia than is halothane. There are very few data of the pathophysiologic background of this observation. Therefore, the authors' aim was to investigate the direct effect of desflurane on calcium release in skinned skeletal muscle fibers.METHODS: For the measurements, single saponin-skinned muscle fiber preparations of BALB/c mice were used. For Ca2+ release experiments, liquid desflurane at 0.6 and 3.5 mm was applied to weakly calcium-buffered solutions with no added Ca2+. Desflurane was diluted in strongly Ca2+-buffered solutions, with [Ca2+] between 3.0 and 24.9 micrometer for [Ca2+]-force relations. Force transients were transformed into Ca2+ transients based on the individual [Ca2+]-force relations. As controls, 30 mm caffeine and equimolar sevoflurane were investigated in the same muscle fibers.RESULTS: At 3.5 mm, desflurane induced peak force transients of 8 +/- 4% (mean +/- SD) of maximal Ca2+-activated force (Tmax). These peak values were significantly smaller than those in the presence of 3.5 mm sevoflurane (24 +/- 10% of Tmax, P < 0.05), and 4 or 5 times smaller than previously reported Ca2+-release-induced force transients by equimolar halothane. Calculated peak Ca2+ transients derived from force transients and induced by 3.5 and 0.6 mm desflurane were significantly smaller than those induced by 30 mm caffeine. The [Ca2+]-force relation was shifted by desflurane, resulting in a Ca2+-sensitizing effect. The maximal Ca2+-activated force was significantly increased by 0.6 mm desflurane in comparison with the control, with no added substance (P </= 0.05).CONCLUSION: Desflurane induces only slight Ca2+ release in skinned skeletal muscle fibers.

AB - BACKGROUND: Desflurane is a weaker trigger of malignant hyperthermia than is halothane. There are very few data of the pathophysiologic background of this observation. Therefore, the authors' aim was to investigate the direct effect of desflurane on calcium release in skinned skeletal muscle fibers.METHODS: For the measurements, single saponin-skinned muscle fiber preparations of BALB/c mice were used. For Ca2+ release experiments, liquid desflurane at 0.6 and 3.5 mm was applied to weakly calcium-buffered solutions with no added Ca2+. Desflurane was diluted in strongly Ca2+-buffered solutions, with [Ca2+] between 3.0 and 24.9 micrometer for [Ca2+]-force relations. Force transients were transformed into Ca2+ transients based on the individual [Ca2+]-force relations. As controls, 30 mm caffeine and equimolar sevoflurane were investigated in the same muscle fibers.RESULTS: At 3.5 mm, desflurane induced peak force transients of 8 +/- 4% (mean +/- SD) of maximal Ca2+-activated force (Tmax). These peak values were significantly smaller than those in the presence of 3.5 mm sevoflurane (24 +/- 10% of Tmax, P < 0.05), and 4 or 5 times smaller than previously reported Ca2+-release-induced force transients by equimolar halothane. Calculated peak Ca2+ transients derived from force transients and induced by 3.5 and 0.6 mm desflurane were significantly smaller than those induced by 30 mm caffeine. The [Ca2+]-force relation was shifted by desflurane, resulting in a Ca2+-sensitizing effect. The maximal Ca2+-activated force was significantly increased by 0.6 mm desflurane in comparison with the control, with no added substance (P </= 0.05).CONCLUSION: Desflurane induces only slight Ca2+ release in skinned skeletal muscle fibers.

KW - Anesthetics, Inhalation/pharmacology

KW - Animals

KW - Calcium/metabolism

KW - Desflurane

KW - Isoflurane/analogs & derivatives

KW - Mice

KW - Mice, Inbred BALB C

KW - Muscle, Skeletal/drug effects

KW - Sarcoplasmic Reticulum/drug effects

U2 - 10.1097/00000542-200009000-00034

DO - 10.1097/00000542-200009000-00034

M3 - Article

C2 - 10969318

SN - 0003-3022

VL - 93

SP - 832

EP - 836

JO - Anesthesiology

JF - Anesthesiology

IS - 3

ER -

Desflurane induces only minor Ca2+ release from the sarcoplasmic reticulum of mammalian skeletal muscle

Abstract

Keywords

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