Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Cinzia Spinato; Aritz Perez Ruiz de Garibay; Magdalena Kierkowicz; Elzbieta Pach; Markus Martincic; Rebecca Klippstein; Maxime Bourgognon; Julie Tzu-Wen Wang; Cécilia Ménard-Moyon; Khuloud T Al-Jamal; Belén Ballesteros; Gerard Tobias; Alberto Bianco

doi:10.1039/c5nr07923c

Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Cinzia Spinato, Aritz Perez Ruiz de Garibay, Magdalena Kierkowicz, Elzbieta Pach, Markus Martincic, Rebecca Klippstein, Maxime Bourgognon, Julie Tzu-Wen Wang, Cécilia Ménard-Moyon, Khuloud T Al-Jamal, Belén Ballesteros, Gerard Tobias, Alberto Bianco

Institute of Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.

Original language	English
Pages (from-to)	12626-12638
Journal	Nanoscale
Volume	8
Issue number	25
Early online date	15 Dec 2015
DOIs	https://doi.org/10.1039/c5nr07923c
Publication status	Published - 6 Jan 2016

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Spinato, C., Perez Ruiz de Garibay, A., Kierkowicz, M., Pach, E., Martincic, M., Klippstein, R., Bourgognon, M., Wang, J. T.-W., Ménard-Moyon, C., Al-Jamal, K. T., Ballesteros, B., Tobias, G., & Bianco, A. (2016). Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy. Nanoscale, 8(25), 12626-12638. https://doi.org/10.1039/c5nr07923c

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title = "Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy",

abstract = "In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.",

author = "Cinzia Spinato and {Perez Ruiz de Garibay}, Aritz and Magdalena Kierkowicz and Elzbieta Pach and Markus Martincic and Rebecca Klippstein and Maxime Bourgognon and Wang, {Julie Tzu-Wen} and C{\'e}cilia M{\'e}nard-Moyon and Al-Jamal, {Khuloud T} and Bel{\'e}n Ballesteros and Gerard Tobias and Alberto Bianco",

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doi = "10.1039/c5nr07923c",

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Spinato, C, Perez Ruiz de Garibay, A, Kierkowicz, M, Pach, E, Martincic, M, Klippstein, R, Bourgognon, M , Wang, JT-W, Ménard-Moyon, C, Al-Jamal, KT, Ballesteros, B, Tobias, G & Bianco, A 2016, 'Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy', Nanoscale, vol. 8, no. 25, pp. 12626-12638. https://doi.org/10.1039/c5nr07923c

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T1 - Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

AU - Spinato, Cinzia

AU - Perez Ruiz de Garibay, Aritz

AU - Kierkowicz, Magdalena

AU - Pach, Elzbieta

AU - Martincic, Markus

AU - Klippstein, Rebecca

AU - Bourgognon, Maxime

AU - Wang, Julie Tzu-Wen

AU - Ménard-Moyon, Cécilia

AU - Al-Jamal, Khuloud T

AU - Ballesteros, Belén

AU - Tobias, Gerard

AU - Bianco, Alberto

PY - 2016/1/6

Y1 - 2016/1/6

N2 - In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.

AB - In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.

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DO - 10.1039/c5nr07923c

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SN - 2040-3364

VL - 8

SP - 12626

EP - 12638

JO - Nanoscale

JF - Nanoscale

IS - 25

ER -

Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Abstract

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