Detection of cell proliferation markers by immunofluorescence staining and microscopy imaging in paraffin-embedded tissue sections

Seda Eminaga, Polakit Teekakirikul, Christine E. Seidman, Jonathan G. Seidman

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

This unit describes a step-by-step protocol to detect and quantify proliferating cells in paraffin-embedded tissue sections. Two well-established markers of proliferation (incorporation of BrdU into newly synthesized DNA and expression of the nuclear protein Ki67) are detected after antigen-retrieval and subsequent immunofluorescence staining and confocal microscopy.

Original languageEnglish
Article number14.25
JournalCurrent Protocols in Molecular Biology
Volume2016
DOIs
Publication statusE-pub ahead of print - 1 Jul 2016

Keywords

  • BrdU
  • Cell proliferation
  • Immunofluorescence
  • Immunohistochemistry
  • Ki67

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