Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

C Biswas, B Kell, C Mant, R J Jewers, J Cason, P Muir, K S Raju, J M Best

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DNA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P = 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.

Original languageEnglish
Pages (from-to)1560-4
Number of pages5
JournalJournal of Clinical Microbiology
Volume35
Issue number6
Publication statusPublished - Jun 1997

Keywords

  • Adult
  • Aged
  • Biomarkers
  • Cervical Intraepithelial Neoplasia/pathology
  • DNA, Viral/analysis
  • Female
  • Humans
  • Middle Aged
  • Oncogene Proteins, Viral/genetics
  • Papillomaviridae/genetics
  • Papillomavirus Infections/pathology
  • Polymerase Chain Reaction/methods
  • RNA, Messenger/analysis
  • RNA, Viral/analysis
  • Sensitivity and Specificity
  • Transcription, Genetic
  • Tumor Virus Infections/pathology
  • Uterine Cervical Neoplasms/pathology
  • Vaginal Smears

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