Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

David Bitto; David J Harvey; Steinar Halldorsson; Katie J Doores; Laura K Pritchard; Juha T Huiskonen; Thomas A Bowden; Max Crispin

doi:10.1007/978-1-4939-2874-3_7

Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

David Bitto, David J Harvey, Steinar Halldorsson, Katie J Doores, Laura K Pritchard, Juha T Huiskonen, Thomas A Bowden, Max Crispin

Infectious Diseases

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

14 Citations (Scopus)

Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Original language	English
Title of host publication	Carbohydrate-Based Vaccines
Subtitle of host publication	Methods and Protocols
Pages	93-121
Number of pages	29
ISBN (Electronic)	978-1-4939-2874-3
DOIs	https://doi.org/10.1007/978-1-4939-2874-3_7
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology
Publisher	Springer New York
Volume	1331
ISSN (Print)	1064-3745

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10.1007/978-1-4939-2874-3_7

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Bitto, D., Harvey, D. J., Halldorsson, S., Doores, K. J., Pritchard, L. K., Huiskonen, J. T., Bowden, T. A., & Crispin, M. (2015). Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility. In Carbohydrate-Based Vaccines: Methods and Protocols (pp. 93-121). (Methods in Molecular Biology; Vol. 1331). https://doi.org/10.1007/978-1-4939-2874-3_7

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title = "Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility",

abstract = "Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.",

author = "David Bitto and Harvey, {David J} and Steinar Halldorsson and Doores, {Katie J} and Pritchard, {Laura K} and Huiskonen, {Juha T} and Bowden, {Thomas A} and Max Crispin",

year = "2015",

doi = "10.1007/978-1-4939-2874-3_7",

language = "English",

isbn = "978-1-4939-2873-6",

series = "Methods in Molecular Biology",

publisher = "Springer New York",

pages = "93--121",

booktitle = "Carbohydrate-Based Vaccines",

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Bitto, D, Harvey, DJ, Halldorsson, S, Doores, KJ, Pritchard, LK, Huiskonen, JT, Bowden, TA & Crispin, M 2015, Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility. in Carbohydrate-Based Vaccines: Methods and Protocols. Methods in Molecular Biology, vol. 1331, pp. 93-121. https://doi.org/10.1007/978-1-4939-2874-3_7

Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility. / Bitto, David; Harvey, David J; Halldorsson, Steinar et al.
Carbohydrate-Based Vaccines: Methods and Protocols. 2015. p. 93-121 (Methods in Molecular Biology; Vol. 1331).

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

TY - CHAP

T1 - Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

AU - Bitto, David

AU - Harvey, David J

AU - Halldorsson, Steinar

AU - Doores, Katie J

AU - Pritchard, Laura K

AU - Huiskonen, Juha T

AU - Bowden, Thomas A

AU - Crispin, Max

PY - 2015

Y1 - 2015

N2 - Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

AB - Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

U2 - 10.1007/978-1-4939-2874-3_7

DO - 10.1007/978-1-4939-2874-3_7

M3 - Other chapter contribution

C2 - 26169737

SN - 978-1-4939-2873-6

T3 - Methods in Molecular Biology

SP - 93

EP - 121

BT - Carbohydrate-Based Vaccines

ER -

Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

Abstract

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