Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

David Bitto, David J Harvey, Steinar Halldorsson, Katie J Doores, Laura K Pritchard, Juha T Huiskonen, Thomas A Bowden, Max Crispin

Research output: Chapter in Book/Report/Conference proceedingOther chapter contributionpeer-review

13 Citations (Scopus)

Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.
Original languageEnglish
Title of host publicationCarbohydrate-Based Vaccines
Subtitle of host publicationMethods and Protocols
Pages93-121
Number of pages29
ISBN (Electronic)978-1-4939-2874-3
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
Volume1331
ISSN (Print)1064-3745

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