Developing Hyperpolarized C-13 Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart

Kilian Weiss; Erika Mariotti; Deborah K. Hill; Matthew R. Orton; Joel T. Dunn; Rodolfo A. Medina; Richard Southworth; Sebastian Kozerke; Thomas R. Eykyn

doi:10.1007/s00723-012-0349-2

Developing Hyperpolarized C-13 Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart

Kilian Weiss, Erika Mariotti, Deborah K. Hill, Matthew R. Orton, Joel T. Dunn, Rodolfo A. Medina, Richard Southworth, Sebastian Kozerke, Thomas R. Eykyn

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Abstract

Hyperpolarized C-13 magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized C-13 investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-C-13] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.

Original language	English
Pages (from-to)	275-288
Number of pages	14
Journal	APPLIED MAGNETIC RESONANCE
Volume	43
Issue number	1-2
Early online date	19 May 2012
DOIs	https://doi.org/10.1007/s00723-012-0349-2
Publication status	Published - Jul 2012

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10.1007/s00723-012-0349-2

PET imaging of cardiac hypoxia and ischaemia with radiocopper complexes
Southworth, R. (Primary Investigator), Blower, P. (Co-Investigator), Marsden, P. (Co-Investigator) & Nagel, E. (Co-Investigator)
BHF British Heart Foundation
1/04/2010 → 10/10/2013
Project: Research

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title = "Developing Hyperpolarized C-13 Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart",

abstract = "Hyperpolarized C-13 magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized C-13 investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-C-13] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.",

author = "Kilian Weiss and Erika Mariotti and Hill, {Deborah K.} and Orton, {Matthew R.} and Dunn, {Joel T.} and Medina, {Rodolfo A.} and Richard Southworth and Sebastian Kozerke and Eykyn, {Thomas R.}",

year = "2012",

month = jul,

doi = "10.1007/s00723-012-0349-2",

language = "English",

volume = "43",

pages = "275--288",

journal = "APPLIED MAGNETIC RESONANCE",

issn = "0937-9347",

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T1 - Developing Hyperpolarized C-13 Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart

AU - Weiss, Kilian

AU - Mariotti, Erika

AU - Hill, Deborah K.

AU - Orton, Matthew R.

AU - Dunn, Joel T.

AU - Medina, Rodolfo A.

AU - Southworth, Richard

AU - Kozerke, Sebastian

AU - Eykyn, Thomas R.

PY - 2012/7

Y1 - 2012/7

N2 - Hyperpolarized C-13 magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized C-13 investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-C-13] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.

AB - Hyperpolarized C-13 magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized C-13 investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-C-13] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.

U2 - 10.1007/s00723-012-0349-2

DO - 10.1007/s00723-012-0349-2

M3 - Article

SN - 0937-9347

VL - 43

SP - 275

EP - 288

JO - APPLIED MAGNETIC RESONANCE

JF - APPLIED MAGNETIC RESONANCE

IS - 1-2

ER -

Developing Hyperpolarized C-13 Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart

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PET imaging of cardiac hypoxia and ischaemia with radiocopper complexes

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