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Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle

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Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. / Perera, Sue; Mankoo, Baljinder; Gautel, Mathias.

In: Journal of Muscle Research and Cell Motility, Vol. 33, No. 2, N/A, 06.2012, p. 107-122.

Research output: Contribution to journalArticle

Harvard

Perera, S, Mankoo, B & Gautel, M 2012, 'Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle', Journal of Muscle Research and Cell Motility, vol. 33, no. 2, N/A, pp. 107-122. https://doi.org/10.1007/s10974-012-9288-7

APA

Perera, S., Mankoo, B., & Gautel, M. (2012). Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. Journal of Muscle Research and Cell Motility, 33(2), 107-122. [N/A]. https://doi.org/10.1007/s10974-012-9288-7

Vancouver

Perera S, Mankoo B, Gautel M. Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. Journal of Muscle Research and Cell Motility. 2012 Jun;33(2):107-122. N/A. https://doi.org/10.1007/s10974-012-9288-7

Author

Perera, Sue ; Mankoo, Baljinder ; Gautel, Mathias. / Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. In: Journal of Muscle Research and Cell Motility. 2012 ; Vol. 33, No. 2. pp. 107-122.

Bibtex Download

@article{d429be52f2fb4d3ca5b0f98c510efe85,
title = "Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle",
abstract = "The striated muscle-specific tripartite motif (TRIM) proteins TRIM63/MURF1, TRIM55/MURF2 and TRIM54/MURF3 can function as E3 ubiquitin ligases in ubiquitin-mediated muscle protein turnover. Despite the well-characterised role of MURF1 in skeletal muscle atrophy, the dynamics of MURF isogene expression in the development and early postnatal adaptation of skeletal muscle is unknown. Here, we show that MURF2 is the isogene most highly expressed in embryonic skeletal muscle at E15.5, with the 50 kDa A isoform predominantly expressed. MURF1 and MURF3 are upregulated only postnatally. Knockdown of MURF2 p50A by isoform-specific siRNA results in delayed myogenic differentiation and myotube formation in vitro, with perturbation of the stable, glutamylated microtubule population. This underscores that MURF2 plays an important role in the earliest stages of skeletal muscle differentiation and myofibrillogenesis. During further development, there is a shift towards the 60 kDa A isoform, which dominates postnatally. Analysis of the fibre-type expression shows that MURF2 A isoforms are predominantly slow-fibre associated, whilst MURF1 is largely excluded from these fibres, and MURF3 is ubiquitously distributed in both type I and II fibres.",
keywords = "MURF, p62, SQSTM1, NBR1, Myofibrils, Microtubules, Skeletal muscle development, Fibre-type differentiation, Slow fibres, SERUM RESPONSE FACTOR, MYOSIN HEAVY-CHAINS, RING-FINGER PROTEIN, MYOGENIC DIFFERENTIATION, SIGNALING PATHWAYS, KINASE DOMAIN, FIBER TYPES, AUTOPHAGY, ATROPHY, MICROTUBULE",
author = "Sue Perera and Baljinder Mankoo and Mathias Gautel",
year = "2012",
month = "6",
doi = "10.1007/s10974-012-9288-7",
language = "English",
volume = "33",
pages = "107--122",
journal = "Journal of Muscle Research and Cell Motility",
issn = "0142-4319",
publisher = "Springer Netherlands",
number = "2",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle

AU - Perera, Sue

AU - Mankoo, Baljinder

AU - Gautel, Mathias

PY - 2012/6

Y1 - 2012/6

N2 - The striated muscle-specific tripartite motif (TRIM) proteins TRIM63/MURF1, TRIM55/MURF2 and TRIM54/MURF3 can function as E3 ubiquitin ligases in ubiquitin-mediated muscle protein turnover. Despite the well-characterised role of MURF1 in skeletal muscle atrophy, the dynamics of MURF isogene expression in the development and early postnatal adaptation of skeletal muscle is unknown. Here, we show that MURF2 is the isogene most highly expressed in embryonic skeletal muscle at E15.5, with the 50 kDa A isoform predominantly expressed. MURF1 and MURF3 are upregulated only postnatally. Knockdown of MURF2 p50A by isoform-specific siRNA results in delayed myogenic differentiation and myotube formation in vitro, with perturbation of the stable, glutamylated microtubule population. This underscores that MURF2 plays an important role in the earliest stages of skeletal muscle differentiation and myofibrillogenesis. During further development, there is a shift towards the 60 kDa A isoform, which dominates postnatally. Analysis of the fibre-type expression shows that MURF2 A isoforms are predominantly slow-fibre associated, whilst MURF1 is largely excluded from these fibres, and MURF3 is ubiquitously distributed in both type I and II fibres.

AB - The striated muscle-specific tripartite motif (TRIM) proteins TRIM63/MURF1, TRIM55/MURF2 and TRIM54/MURF3 can function as E3 ubiquitin ligases in ubiquitin-mediated muscle protein turnover. Despite the well-characterised role of MURF1 in skeletal muscle atrophy, the dynamics of MURF isogene expression in the development and early postnatal adaptation of skeletal muscle is unknown. Here, we show that MURF2 is the isogene most highly expressed in embryonic skeletal muscle at E15.5, with the 50 kDa A isoform predominantly expressed. MURF1 and MURF3 are upregulated only postnatally. Knockdown of MURF2 p50A by isoform-specific siRNA results in delayed myogenic differentiation and myotube formation in vitro, with perturbation of the stable, glutamylated microtubule population. This underscores that MURF2 plays an important role in the earliest stages of skeletal muscle differentiation and myofibrillogenesis. During further development, there is a shift towards the 60 kDa A isoform, which dominates postnatally. Analysis of the fibre-type expression shows that MURF2 A isoforms are predominantly slow-fibre associated, whilst MURF1 is largely excluded from these fibres, and MURF3 is ubiquitously distributed in both type I and II fibres.

KW - MURF

KW - p62

KW - SQSTM1

KW - NBR1

KW - Myofibrils

KW - Microtubules

KW - Skeletal muscle development

KW - Fibre-type differentiation

KW - Slow fibres

KW - SERUM RESPONSE FACTOR

KW - MYOSIN HEAVY-CHAINS

KW - RING-FINGER PROTEIN

KW - MYOGENIC DIFFERENTIATION

KW - SIGNALING PATHWAYS

KW - KINASE DOMAIN

KW - FIBER TYPES

KW - AUTOPHAGY

KW - ATROPHY

KW - MICROTUBULE

U2 - 10.1007/s10974-012-9288-7

DO - 10.1007/s10974-012-9288-7

M3 - Article

VL - 33

SP - 107

EP - 122

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

SN - 0142-4319

IS - 2

M1 - N/A

ER -

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