Diacylglycerol at the inner nuclear membrane fuels nuclear envelope expansion in closed mitosis

Sherman Foo, Amaury Cazenave-Gassiot, Markus Wenk, Snezhana Oliferenko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Nuclear envelope (NE) expansion must be controlled to maintain nuclear shape and function. The nuclear membrane expands massively during closed mitosis, enabling chromosome segregation within an intact NE. Phosphatidic acid (PA) and diacylglycerol (DG) can both serve as biosynthetic precursors for membrane lipid synthesis. How they are regulated in time and space and what the implications are of changes in their flux for mitotic fidelity are largely unknown. Using genetically encoded PA andDGprobes,we show that DG is depleted from the inner nuclear membrane during mitosis in the fission yeast Schizosaccharomyces pombe, but PA does not accumulate, indicating that it is rerouted to membrane synthesis. We demonstrate that DG-to-PA conversion catalyzed by the diacylglycerol kinase Dgk1 (also known as Ptp4) and direct glycerophospholipid synthesis from DG by diacylglycerol cholinephosphotransferase/ ethanolaminephosphotransferase Ept1 reinforce NE expansion. We conclude that DG consumption through both the de novo pathway and the Kennedy pathway fuels a spike in glycerophospholipid biosynthesis, controlling NE expansion and, ultimately, mitotic fidelity.

Original languageEnglish
Article numberjcs260568
JournalJournal of cell science
Volume136
Issue number3
DOIs
Publication statusPublished - 2 Feb 2023

Keywords

  • Nuclear Envelope
  • Lipid Metabolism
  • Diacylglycerol
  • CLOSED MITOSIS

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