Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat

TY - JOUR

T1 - Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat

AU - Palmer, A M

AU - Thomas, C R

AU - Gopaul, N

AU - Dhir, S

AU - Anggård, E E

AU - Poston, L

AU - Tribe, R M

PY - 1998/1

Y1 - 1998/1

N2 - Impaired endothelium-dependent relaxation could underlie many of the vascular complications associated with insulin-dependent diabetes mellitus, and may be mediated by increased oxidative stress. The effect of antioxidants on vascular endothelial function and oxidative stress of streptozotocin-diabetic rats was assessed by dietary supplementation with vitamins E and C. Diabetic (i.v. streptozotocin, 45 mg/kg) male Sprague-Dawley rats were fed one of six supplemented diets containing 75.9, 250, or 500 mg vitamin E/kg chow, 250 mg vitamin C/kg H2O, 250 mg vitamin E/kg chow plus 250 mg vitamin C/kg H2O, or chow deficient in vitamin E, and then compared to standard-fed control rats. After 4 weeks, small mesenteric arteries were dissected and mounted on a small vessel myograph, concentration response curves were then constructed to noradrenaline, acetylcholine and sodium nitroprusside. Acetylcholine-mediated relaxation was impaired in arteries from diabetic rats (pEC50 6.701+/-SEM 0.120, n = 8) compared to controls (7.386+/-0.078, n = 6; p

AB - Impaired endothelium-dependent relaxation could underlie many of the vascular complications associated with insulin-dependent diabetes mellitus, and may be mediated by increased oxidative stress. The effect of antioxidants on vascular endothelial function and oxidative stress of streptozotocin-diabetic rats was assessed by dietary supplementation with vitamins E and C. Diabetic (i.v. streptozotocin, 45 mg/kg) male Sprague-Dawley rats were fed one of six supplemented diets containing 75.9, 250, or 500 mg vitamin E/kg chow, 250 mg vitamin C/kg H2O, 250 mg vitamin E/kg chow plus 250 mg vitamin C/kg H2O, or chow deficient in vitamin E, and then compared to standard-fed control rats. After 4 weeks, small mesenteric arteries were dissected and mounted on a small vessel myograph, concentration response curves were then constructed to noradrenaline, acetylcholine and sodium nitroprusside. Acetylcholine-mediated relaxation was impaired in arteries from diabetic rats (pEC50 6.701+/-SEM 0.120, n = 8) compared to controls (7.386+/-0.078, n = 6; p

KW - Animals

KW - Antioxidants

KW - Ascorbic Acid

KW - Diabetes Mellitus, Experimental

KW - Dietary Supplements

KW - Dinoprost

KW - Drug Interactions

KW - Endothelium, Vascular

KW - F2-Isoprostanes

KW - Lipid Peroxidation

KW - Liver

KW - Male

KW - Mesenteric Arteries

KW - Muscle Relaxation

KW - Oxidative Stress

KW - Rats

KW - Rats, Sprague-Dawley

KW - Vitamin E

U2 - 10.1007/s001250050883

DO - 10.1007/s001250050883

M3 - Article

C2 - 9498647

SN - 0012-186X

VL - 41

SP - 148

EP - 156

JO - Diabetologia

JF - Diabetologia

IS - 2

ER -