Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat

A M Palmer, C R Thomas, N Gopaul, S Dhir, E E Anggård, L Poston, R M Tribe

Research output: Contribution to journalArticlepeer-review

102 Citations (Scopus)

Abstract

Impaired endothelium-dependent relaxation could underlie many of the vascular complications associated with insulin-dependent diabetes mellitus, and may be mediated by increased oxidative stress. The effect of antioxidants on vascular endothelial function and oxidative stress of streptozotocin-diabetic rats was assessed by dietary supplementation with vitamins E and C. Diabetic (i.v. streptozotocin, 45 mg/kg) male Sprague-Dawley rats were fed one of six supplemented diets containing 75.9, 250, or 500 mg vitamin E/kg chow, 250 mg vitamin C/kg H2O, 250 mg vitamin E/kg chow plus 250 mg vitamin C/kg H2O, or chow deficient in vitamin E, and then compared to standard-fed control rats. After 4 weeks, small mesenteric arteries were dissected and mounted on a small vessel myograph, concentration response curves were then constructed to noradrenaline, acetylcholine and sodium nitroprusside. Acetylcholine-mediated relaxation was impaired in arteries from diabetic rats (pEC50 6.701+/-SEM 0.120, n = 8) compared to controls (7.386+/-0.078, n = 6; p
Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalDiabetologia
Volume41
Issue number2
DOIs
Publication statusPublished - Jan 1998

Keywords

  • Animals
  • Antioxidants
  • Ascorbic Acid
  • Diabetes Mellitus, Experimental
  • Dietary Supplements
  • Dinoprost
  • Drug Interactions
  • Endothelium, Vascular
  • F2-Isoprostanes
  • Lipid Peroxidation
  • Liver
  • Male
  • Mesenteric Arteries
  • Muscle Relaxation
  • Oxidative Stress
  • Rats
  • Rats, Sprague-Dawley
  • Vitamin E

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