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Dietary Trivalent Chromium Exposure Up-Regulates Lipid Metabolism in Coral Trout: The Evidence From Transcriptome Analysis

Research output: Contribution to journalArticlepeer-review

Lu Wei, Yu Li, Hengzhen Ye, Juan Xiao, Christer Hogstrand, Iain Green, Zhiqiang Guo, Dong Han

Original languageEnglish
Article number640898
JournalFrontiers in Physiology
Volume12
DOIs
Published25 Feb 2021

Bibliographical note

Funding Information: The research was financially supported by the National Key Research and Development Program of China (2018YFD0900704), the Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-06), the National Natural Science Foundation of China (31872574, 41866003, 31960731, 31672670, and 31972771), the Scientific Research Start-up fund of Hainan University (KYQD(ZR)1803), the Natural Science Foundation of Hainan Province, and the Hainan Province Postdoctoral Research for Postdoctoral funding for LW. Publisher Copyright: © Copyright © 2021 Wei, Li, Ye, Xiao, Hogstrand, Green, Guo and Han. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors

Abstract

Diet quality greatly affects an animal’s performance and metabolism. Despite the fact that trivalent chromium [Cr(III)] is considered an essential element and is widely used in nutritional supplements for animals and humans, the potential toxicity of Cr(III) is unclear. Here, liver transcriptome sequencing was performed on coral trout (Plectropomus leopardus) exposed to 200 mg kg–1 of dietary organic Cr(III) [as chromium picolinate (CrPic)] for 8 weeks. One-hundred-and thirteen differentially expressed genes (DEGs) were identified in response to Cr(III) stress, in comparison to the control, including 31 up-regulated and 82 down-regulated DEGs. Clusters of Orthologous Groups of proteins (COG) classifies DEGs into 15 functional categories, with the predominant category being related to lipid transport and metabolism (9.73%). The Kyoto Encyclopedia of Genes and Genomes (KEGG) assigned DEGs to six major categories with robust DEGs as part of the lipid metabolism pathway (18.58%). Moreover, KEGG functional enrichment analysis showed that these DEGs are primarily related to steroid biosynthesis, terpenoid backbone biosynthesis, and steroid hormone biosynthesis pathways, of which steroid biosynthesis was the most significant pathway, and 12 key up-regulated DEGs (dhcr7, dhcr24, ebp, lss, msmo1, sqle, cyp51, tm7sf2, sc5dl, fdft1, nsdhl, and hsd17b7) were found for steroid biosynthesis pathways. To validate the RNA sequencing data using quantitative real-time PCR (qRT-PCR), qRT-PCR results indicate that the expression of genes encoding HMGCR, TM7SF2, TRYP2, CTRL, EBP, LSS, and CYP51 were induced, while those encoding THRSP, LCE, and MCM5 were reduced, consistent with RNA-seq results. This findings provides the first evidence that a long-term high dose of Cr(III) intake causes lipid metabolism disorder and potential toxicity in fish. Cautious health risk assessment of dietary Cr(III) intake is therefore highly recommended for the commercial and/or natural diets of aquatic animals, which has previously largely been ignored.

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