TY - JOUR
T1 - Differential cellular responses to FDA-approved nanomedicines
T2 - an exploration of albumin-based nanocarriers and liposomes in protein corona formation
AU - Putri, Athika Darumas
AU - Hsu, Ming-Jen
AU - Han, Chia-Li
AU - Chao, Fang-Ching
AU - Hsu, Chun-Hua
AU - Lorenz, Christian D.
AU - Hsieh, Chien-Ming
N1 - Funding Information:
This work was financially supported by a grant from the Ministry of Science and Technology (MOST109-2221-E-038-001-MY3 and MOST110-2113-M038-001-MY3) and the National Science and Technology Council (NSTC112-2221-E-038-014), Taiwan. Mass spectrometry data were acquired at the Common Mass Spectrometry Facilities located at the Department of Chemistry, National Taiwan University (Taiwan). The authors thank Dr Bayu Tri Murti (Semarang College of Pharmaceutical Sciences (STIFAR) Semarang, Indonesia) for the helpful schematic illustration. The assistance of Dr Pu-Sheng Wei (Taipei Medical University) in confocal imaging measurements is greatly acknowledged. The authors gratefully thank the Core Facility of Taipei Medical University for facilitating the instrument use. The authors also thank The National Center for High-Performance Computing, Hsinchu City, Taiwan for providing the necessary computational resources.
Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/9/29
Y1 - 2023/9/29
N2 - Albumin nanoparticles (NPs) and PEGylated liposomes have garnered tremendous interest as therapeutic drug carriers due to their unique physicochemical properties. These unique properties also have significant effects on the composition and structure of the protein corona formed around these NPs in a biological environment. Herein, protein corona formation on albumin NPs and liposomes was simultaneously evaluated through in vitro and simulation studies. The sizes of both types of NPs increased with more negatively charged interfaces upon being introduced into fetal bovine serum. Gel electrophoresis and label-free quantitative proteomics were performed to identify proteins recruited to the hard corona, and fewer proteins were found in albumin NPs than in liposomes, which is in accordance with isothermal titration calorimetry. The cellular uptake efficiency of the two NPs significantly differed in different serum concentrations, which was further scrutinized by loading an anticancer compound into albumin NPs. The presence of the hard protein corona increased the cellular uptake of albumin NPs in comparison with liposomes. In our simulation study, a specific receptor present in the membrane was greatly attracted to the albumin–apolipoprotein E complex. Overall, this study not only evaluated protein corona formation on albumin NPs, but also made promising advancements toward albumin- and liposome-based therapeutic systems.
AB - Albumin nanoparticles (NPs) and PEGylated liposomes have garnered tremendous interest as therapeutic drug carriers due to their unique physicochemical properties. These unique properties also have significant effects on the composition and structure of the protein corona formed around these NPs in a biological environment. Herein, protein corona formation on albumin NPs and liposomes was simultaneously evaluated through in vitro and simulation studies. The sizes of both types of NPs increased with more negatively charged interfaces upon being introduced into fetal bovine serum. Gel electrophoresis and label-free quantitative proteomics were performed to identify proteins recruited to the hard corona, and fewer proteins were found in albumin NPs than in liposomes, which is in accordance with isothermal titration calorimetry. The cellular uptake efficiency of the two NPs significantly differed in different serum concentrations, which was further scrutinized by loading an anticancer compound into albumin NPs. The presence of the hard protein corona increased the cellular uptake of albumin NPs in comparison with liposomes. In our simulation study, a specific receptor present in the membrane was greatly attracted to the albumin–apolipoprotein E complex. Overall, this study not only evaluated protein corona formation on albumin NPs, but also made promising advancements toward albumin- and liposome-based therapeutic systems.
UR - http://www.scopus.com/inward/record.url?scp=85175527838&partnerID=8YFLogxK
U2 - 10.1039/D3NR04862D
DO - 10.1039/D3NR04862D
M3 - Article
SN - 2040-3364
VL - 15
SP - 17825
EP - 17838
JO - Nanoscale
JF - Nanoscale
IS - 44
ER -