Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

Ehsan Soltaninejad; Mohammad Hossein Nicknam; Mohsen Nafar; Pedram Ahmadpoor; Fatemeh Pourrezagholi; Mohammad Hossein Sharbafi; Morteza Hosseinzadeh; Farshad Foroughi; Mir Saeed Yekaninejad; Tayyeb Bahrami; Ehsan Sharif-Paghaleh; Aliakbar Amirzargar

doi:10.1016/j.trim.2015.05.002

Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

Ehsan Soltaninejad, Mohammad Hossein Nicknam, Mohsen Nafar, Pedram Ahmadpoor, Fatemeh Pourrezagholi, Mohammad Hossein Sharbafi, Morteza Hosseinzadeh, Farshad Foroughi, Mir Saeed Yekaninejad, Tayyeb Bahrami, Ehsan Sharif-Paghaleh, Aliakbar Amirzargar

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).

METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.

RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.

CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Transplant Immunology
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.trim.2015.05.002
Publication status	Published - Sept 2015

Keywords

Acute Disease
Adult
Aged
Biopsy
Female
Gene Expression Regulation
Graft Rejection
Humans
Kidney
Kidney Transplantation
Male
MicroRNAs
Middle Aged
T-Lymphocytes

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10.1016/j.trim.2015.05.002

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Soltaninejad, E., Nicknam, M. H., Nafar, M., Ahmadpoor, P., Pourrezagholi, F., Sharbafi, M. H., Hosseinzadeh, M., Foroughi, F., Yekaninejad, M. S., Bahrami, T., Sharif-Paghaleh, E., & Amirzargar, A. (2015). Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection. Transplant Immunology, 33(1), 1-6. https://doi.org/10.1016/j.trim.2015.05.002

@article{271ee2cf293f4af5acaa8dea0f1aa8a5,

title = "Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection",

abstract = "BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.",

keywords = "Acute Disease, Adult, Aged, Biopsy, Female, Gene Expression Regulation, Graft Rejection, Humans, Kidney, Kidney Transplantation, Male, MicroRNAs, Middle Aged, T-Lymphocytes",

author = "Ehsan Soltaninejad and Nicknam, {Mohammad Hossein} and Mohsen Nafar and Pedram Ahmadpoor and Fatemeh Pourrezagholi and Sharbafi, {Mohammad Hossein} and Morteza Hosseinzadeh and Farshad Foroughi and Yekaninejad, {Mir Saeed} and Tayyeb Bahrami and Ehsan Sharif-Paghaleh and Aliakbar Amirzargar",

year = "2015",

month = sep,

doi = "10.1016/j.trim.2015.05.002",

language = "English",

volume = "33",

pages = "1--6",

journal = "Transplant Immunology",

issn = "0966-3274",

publisher = "Elsevier",

number = "1",

}

Soltaninejad, E, Nicknam, MH, Nafar, M, Ahmadpoor, P, Pourrezagholi, F, Sharbafi, MH, Hosseinzadeh, M, Foroughi, F, Yekaninejad, MS, Bahrami, T, Sharif-Paghaleh, E & Amirzargar, A 2015, 'Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection', Transplant Immunology, vol. 33, no. 1, pp. 1-6. https://doi.org/10.1016/j.trim.2015.05.002

TY - JOUR

T1 - Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

AU - Soltaninejad, Ehsan

AU - Nicknam, Mohammad Hossein

AU - Nafar, Mohsen

AU - Ahmadpoor, Pedram

AU - Pourrezagholi, Fatemeh

AU - Sharbafi, Mohammad Hossein

AU - Hosseinzadeh, Morteza

AU - Foroughi, Farshad

AU - Yekaninejad, Mir Saeed

AU - Bahrami, Tayyeb

AU - Sharif-Paghaleh, Ehsan

AU - Amirzargar, Aliakbar

PY - 2015/9

Y1 - 2015/9

N2 - BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.

AB - BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.

KW - Acute Disease

KW - Adult

KW - Aged

KW - Biopsy

KW - Female

KW - Gene Expression Regulation

KW - Graft Rejection

KW - Humans

KW - Kidney

KW - Kidney Transplantation

KW - Male

KW - MicroRNAs

KW - Middle Aged

KW - T-Lymphocytes

U2 - 10.1016/j.trim.2015.05.002

DO - 10.1016/j.trim.2015.05.002

M3 - Article

C2 - 26002284

SN - 0966-3274

VL - 33

SP - 1

EP - 6

JO - Transplant Immunology

JF - Transplant Immunology

IS - 1

ER -

Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

Abstract

Keywords

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