Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

Ehsan Soltaninejad, Mohammad Hossein Nicknam, Mohsen Nafar, Pedram Ahmadpoor, Fatemeh Pourrezagholi, Mohammad Hossein Sharbafi, Morteza Hosseinzadeh, Farshad Foroughi, Mir Saeed Yekaninejad, Tayyeb Bahrami, Ehsan Sharif-Paghaleh, Aliakbar Amirzargar

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA).

    METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA.

    RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients.

    CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.

    Original languageEnglish
    Pages (from-to)1-6
    Number of pages6
    JournalTransplant Immunology
    Volume33
    Issue number1
    DOIs
    Publication statusPublished - Sept 2015

    Keywords

    • Acute Disease
    • Adult
    • Aged
    • Biopsy
    • Female
    • Gene Expression Regulation
    • Graft Rejection
    • Humans
    • Kidney
    • Kidney Transplantation
    • Male
    • MicroRNAs
    • Middle Aged
    • T-Lymphocytes

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