Differential role for PAK1 and PAK4 during the invadopodia lifecycle

Nicole Nicholas; Aikaterini Pipili; Michaela S. Lesjak; Claire M. Wells

doi:10.1080/21541248.2017.1295830

Differential role for PAK1 and PAK4 during the invadopodia lifecycle

Nicole Nicholas, Aikaterini Pipili, Michaela S. Lesjak, Claire M. Wells^*

^*Corresponding author for this work

GSTT Guy's and St Thomas' NHS Foundation Trust

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

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Abstract

PAK1 and PAK4 are members of the p-21 activated kinase family of serine/threonine kinases. PAK1 has previously been implicated in both the formation and disassembly of invasive cell protrusions, termed invadopodia. We recently reported a novel role for PAK4 during invadopodia maturation and confirmed a specific role for PAK1 in invadopodia formation; findings we will review here. Moreover, we found that PAK4 induction of maturation is delivered via interaction with the RhoA regulator PDZ-RhoGEF. We can now reveal that loss of PAK4 expression leads to changes in invadopodia dynamics. Ultimately we propose that PAK4 but not PAK1 is a key mediator of RhoA activity and provide further evidence that modulation of PAK4 expression levels leads to changes in RhoA activity.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Small GTPases
Early online date	17 Mar 2017
DOIs	https://doi.org/10.1080/21541248.2017.1295830
Publication status	E-pub ahead of print - 17 Mar 2017

Keywords

invadopodia
PAK
RhoA

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10.1080/21541248.2017.1295830

Differential role for PAK1_NICHOLAS_Publishedonline17March2017_GREEN AAMAccepted author manuscript, 5.07 MB

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title = "Differential role for PAK1 and PAK4 during the invadopodia lifecycle",

abstract = "PAK1 and PAK4 are members of the p-21 activated kinase family of serine/threonine kinases. PAK1 has previously been implicated in both the formation and disassembly of invasive cell protrusions, termed invadopodia. We recently reported a novel role for PAK4 during invadopodia maturation and confirmed a specific role for PAK1 in invadopodia formation; findings we will review here. Moreover, we found that PAK4 induction of maturation is delivered via interaction with the RhoA regulator PDZ-RhoGEF. We can now reveal that loss of PAK4 expression leads to changes in invadopodia dynamics. Ultimately we propose that PAK4 but not PAK1 is a key mediator of RhoA activity and provide further evidence that modulation of PAK4 expression levels leads to changes in RhoA activity.",

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AU - Nicholas, Nicole

AU - Pipili, Aikaterini

AU - Lesjak, Michaela S.

AU - Wells, Claire M.

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AB - PAK1 and PAK4 are members of the p-21 activated kinase family of serine/threonine kinases. PAK1 has previously been implicated in both the formation and disassembly of invasive cell protrusions, termed invadopodia. We recently reported a novel role for PAK4 during invadopodia maturation and confirmed a specific role for PAK1 in invadopodia formation; findings we will review here. Moreover, we found that PAK4 induction of maturation is delivered via interaction with the RhoA regulator PDZ-RhoGEF. We can now reveal that loss of PAK4 expression leads to changes in invadopodia dynamics. Ultimately we propose that PAK4 but not PAK1 is a key mediator of RhoA activity and provide further evidence that modulation of PAK4 expression levels leads to changes in RhoA activity.

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Differential role for PAK1 and PAK4 during the invadopodia lifecycle

Abstract

Keywords

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