TY - JOUR
T1 - Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
AU - Pedraza-Arevalo, Sergio
AU - Cujba, Ana Maria
AU - Alvarez-Fallas, Mario Enrique
AU - Sancho, Rocio
N1 - Publisher Copyright:
© 2022 The Authors.
PY - 2022/9/16
Y1 - 2022/9/16
N2 - Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).
AB - Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).
KW - Cell differentiation
KW - Developmental biology
KW - Metabolism
KW - Organoids
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=85137712772&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2022.101656
DO - 10.1016/j.xpro.2022.101656
M3 - Article
C2 - 36092820
AN - SCOPUS:85137712772
SN - 2666-1667
VL - 3
SP - 101656
JO - STAR Protocols
JF - STAR Protocols
IS - 3
ER -
