Abstract
Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.
Original language | English |
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Pages (from-to) | 709-712 |
Number of pages | 4 |
Journal | European Biophysics Journal |
Volume | 35 |
Issue number | 8 |
DOIs | |
Publication status | Published - Oct 2006 |
Keywords
- Animals
- Kinetics
- Ligands
- Neurotensin
- Rats
- Receptors, Neurotensin
- Surface Plasmon Resonance