Direct analysis of a GPCR-agonist interaction by surface plasmon resonance

Peter J Harding, Timothy C Hadingham, James M McDonnell, Anthony Watts

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.
Original languageEnglish
Pages (from-to)709-712
Number of pages4
JournalEuropean Biophysics Journal
Volume35
Issue number8
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Animals
  • Kinetics
  • Ligands
  • Neurotensin
  • Rats
  • Receptors, Neurotensin
  • Surface Plasmon Resonance

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