Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

Peter Gawne, Francis Man, Philip Blower, Rafael T. M. de Rosales*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

53 Citations (Scopus)
270 Downloads (Pure)


The arrival of cell-based therapies is a revolution in medicine. However, its safe clinical application in a rational manner depends on reliable, clinically applicable methods for determining the fate and trafficking of therapeutic cells in vivo using medical imaging techniques known as in vivo cell tracking. Radionuclide imaging using single photon emission computed tomography (SPECT) or positron emission tomography (PET) has several advantages over other imaging modalities for cell tracking because of its high sensitivity (requiring low amounts of probe per cell for imaging) and whole-body quantitative imaging capability using clinically available scanners. For cell tracking with radionuclides, ex vivo direct cell radiolabeling, that is, radiolabeling cells before their administration, is the simplest and most robust method, allowing labeling of any cell type without the need for genetic modification. This Review covers the development and application of direct cell radiolabeling probes utilizing a variety of chemical approaches: organic and inorganic/coordination (radio)chemistry, nanomaterials, and biochemistry. We describe the key early developments and the most recent advances in the field, identifying advantages and disadvantages of the different approaches and informing future development and choice of methods for clinical and preclinical application.

Original languageEnglish
Pages (from-to)10266-10318
Number of pages53
JournalChemical Reviews
Issue number11
Early online date12 May 2022
Publication statusPublished - 8 Jun 2022


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