Disrupted Peyer’s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus

Silvia Cellone Trevelin; Suzy Pickering; Katrina Todd; Cynthia Bishop; Michael Pitcher; Jose Garrido Mesa; Lucia Montorsi; Filomena  Spada; Nedyalko Petrov; Anna Green; Manu Shankar-Hari; Stuart Neil; Jo Spencer

Disrupted Peyer’s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus

Silvia Cellone Trevelin^*, Suzy Pickering, Katrina Todd, Cynthia Bishop, Michael Pitcher, Jose Garrido Mesa, Lucia Montorsi, Filomena Spada, Nedyalko Petrov, Anna Green, Manu Shankar-Hari, Stuart Neil, Jo Spencer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Downloads (Pure)

Abstract

Abstract
Confirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis.

Original language	English
Journal	Frontiers in Immunity- sub specialisation, Mucosal Immunity
Publication status	Accepted/In press - 27 Jan 2022

Access to Document

Trevelin et alAccepted author manuscript, 23.1 MBLicence: Unspecified

Cite this

Cellone Trevelin, S., Pickering, S., Todd, K., Bishop, C., Pitcher, M., Garrido Mesa, J., Montorsi, L., Spada, F., Petrov, N., Green, A., Shankar-Hari, M., Neil, S., & Spencer, J. (Accepted/In press). Disrupted Peyer’s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus. Frontiers in Immunity- sub specialisation, Mucosal Immunity.

@article{93cdab4131c5460f9dcb3c468c4a6714,

title = "Disrupted Peyer{\textquoteright}s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus",

abstract = "AbstractConfirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer{\textquoteright}s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis.",

author = "{Cellone Trevelin}, Silvia and Suzy Pickering and Katrina Todd and Cynthia Bishop and Michael Pitcher and {Garrido Mesa}, Jose and Lucia Montorsi and Filomena Spada and Nedyalko Petrov and Anna Green and Manu Shankar-Hari and Stuart Neil and Jo Spencer",

year = "2022",

month = jan,

day = "27",

language = "English",

journal = "Frontiers in Immunity- sub specialisation, Mucosal Immunity",

}

TY - JOUR

T1 - Disrupted Peyer’s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus

AU - Cellone Trevelin, Silvia

AU - Pickering, Suzy

AU - Todd, Katrina

AU - Bishop, Cynthia

AU - Pitcher, Michael

AU - Garrido Mesa, Jose

AU - Montorsi, Lucia

AU - Spada, Filomena

AU - Petrov, Nedyalko

AU - Green, Anna

AU - Shankar-Hari, Manu

AU - Neil, Stuart

AU - Spencer, Jo

PY - 2022/1/27

Y1 - 2022/1/27

N2 - AbstractConfirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis.

AB - AbstractConfirmed SARS-coronavirus-2 infection with gastrointestinal symptoms and changes in microbiota associated with coronavirus disease 2019 (COVID-19) severity have been previously reported, but the disease impact on the architecture and cellularity of ileal Peyer’s patches (PP) remains unknown. Here we analysed post-mortem tissues from throughout the gastrointestinal (GI) tract of patients who died with COVID-19. When virus was detected by PCR in the GI tract, immunohistochemistry identified virus in epithelium and lamina propria macrophages, but not in lymphoid tissues. Immunohistochemistry and imaging mass cytometry (IMC) analysis of ileal PP revealed depletion of germinal centres (GC), disruption of B cell/T cell zonation and decreased potential B and T cell interaction and lower nuclear density in COVID-19 patients. This occurred independent of the local viral levels. The changes in PP demonstrate that the ability to mount an intestinal immune response is compromised in severe COVID-19, which could contribute to observed dysbiosis.

M3 - Article

JO - Frontiers in Immunity- sub specialisation, Mucosal Immunity

JF - Frontiers in Immunity- sub specialisation, Mucosal Immunity

ER -

Disrupted Peyer’s patch microanatomy in COVID-19 including germinal centre atrophy independent of local virus

Abstract

Access to Document

Fingerprint

Cite this