Dissection of effector pathways in the host-versus-graft response to bone marrow transplantation

L. Weng; C. Addey; A. N. Warrens; J. Dyson; J. G. Chai; F. Dazzi

doi:10.1097/TP.0b013e3181872878

Dissection of effector pathways in the host-versus-graft response to bone marrow transplantation

L. Weng, C. Addey, A. N. Warrens, J. Dyson, J. G. Chai, F. Dazzi

Research output: Contribution to journal › Article › peer-review

Abstract

We have dissected the role of the primary cytotoxic pathways Fas-FasL and perforin-granzyme in host-versus-graft reactions after allogeneic bone marrow transplantation. Sublethally irradiated female recipient mice deficient for FasL (B6.gld) or perforin (B6.prf-/-) were transplanted with bone marrow from B6 male donors. Donor engraftment in B6.prf-/- recipients was higher when compared with B6.gld, particularly when assessed by in vivo killing, demonstrating the importance of the perforin pathway over the Fas-FasL pathway. In the absence of both pathways, however, donor bone marrow engraftment was not fully restored identifying a role for an additional pathway(s) in the host-versus-graft response.

Original language	English
Article number	N/A
Pages (from-to)	1311-1314
Number of pages	4
Journal	Transplantation
Volume	86
Issue number	9
DOIs	https://doi.org/10.1097/TP.0b013e3181872878
Publication status	Published - 15 Nov 2008

Keywords

Animals Antigens, CD95/genetics/*metabolism Bone Marrow Transplantation/*immunology/*physiology Cytotoxicity, Immunologic Fas Ligand Protein/genetics/*metabolism Female Gene Expression Regulation/radiation effects H-Y Antigen/metabolism Hematopoietic Stem Cell Transplantation Host vs Graft Reaction/genetics/*physiology Male Mice Mice, Inbred C57BL Perforin/genetics/*metabolism Signal Transduction/genetics/*physiology Spleen/cytology/transplantation

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title = "Dissection of effector pathways in the host-versus-graft response to bone marrow transplantation",

abstract = "We have dissected the role of the primary cytotoxic pathways Fas-FasL and perforin-granzyme in host-versus-graft reactions after allogeneic bone marrow transplantation. Sublethally irradiated female recipient mice deficient for FasL (B6.gld) or perforin (B6.prf-/-) were transplanted with bone marrow from B6 male donors. Donor engraftment in B6.prf-/- recipients was higher when compared with B6.gld, particularly when assessed by in vivo killing, demonstrating the importance of the perforin pathway over the Fas-FasL pathway. In the absence of both pathways, however, donor bone marrow engraftment was not fully restored identifying a role for an additional pathway(s) in the host-versus-graft response.",

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author = "L. Weng and C. Addey and Warrens, {A. N.} and J. Dyson and Chai, {J. G.} and F. Dazzi",

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doi = "10.1097/TP.0b013e3181872878",

language = "English",

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T1 - Dissection of effector pathways in the host-versus-graft response to bone marrow transplantation

AU - Weng, L.

AU - Addey, C.

AU - Warrens, A. N.

AU - Dyson, J.

AU - Chai, J. G.

AU - Dazzi, F.

PY - 2008/11/15

Y1 - 2008/11/15

N2 - We have dissected the role of the primary cytotoxic pathways Fas-FasL and perforin-granzyme in host-versus-graft reactions after allogeneic bone marrow transplantation. Sublethally irradiated female recipient mice deficient for FasL (B6.gld) or perforin (B6.prf-/-) were transplanted with bone marrow from B6 male donors. Donor engraftment in B6.prf-/- recipients was higher when compared with B6.gld, particularly when assessed by in vivo killing, demonstrating the importance of the perforin pathway over the Fas-FasL pathway. In the absence of both pathways, however, donor bone marrow engraftment was not fully restored identifying a role for an additional pathway(s) in the host-versus-graft response.

AB - We have dissected the role of the primary cytotoxic pathways Fas-FasL and perforin-granzyme in host-versus-graft reactions after allogeneic bone marrow transplantation. Sublethally irradiated female recipient mice deficient for FasL (B6.gld) or perforin (B6.prf-/-) were transplanted with bone marrow from B6 male donors. Donor engraftment in B6.prf-/- recipients was higher when compared with B6.gld, particularly when assessed by in vivo killing, demonstrating the importance of the perforin pathway over the Fas-FasL pathway. In the absence of both pathways, however, donor bone marrow engraftment was not fully restored identifying a role for an additional pathway(s) in the host-versus-graft response.

KW - Animals Antigens, CD95/genetics/metabolism Bone Marrow Transplantation/immunology/physiology Cytotoxicity, Immunologic Fas Ligand Protein/genetics/metabolism Female Gene Expression Regulation/radiation effects H-Y Antigen/metabolism Hematopoietic Stem Cel

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DO - 10.1097/TP.0b013e3181872878

M3 - Article

SN - 1534-6080

VL - 86

SP - 1311

EP - 1314

JO - Transplantation

JF - Transplantation

IS - 9

M1 - N/A

ER -

Dissection of effector pathways in the host-versus-graft response to bone marrow transplantation

Abstract

Keywords

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