DNA-protein interaction dynamics at the Lamin B2 replication origin http://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.973337

Luca Puzzi; Laura Marchetti; Fiorenzo A. Peverali; Giuseppe Biamonti; Mauro Giacca

doi:10.4161/15384101.2014.973337

DNA-protein interaction dynamics at the Lamin B2 replication origin http://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.973337

Luca Puzzi, Laura Marchetti, Fiorenzo A. Peverali, Giuseppe Biamonti, Mauro Giacca^*

^*Corresponding author for this work

Cardiovascular Medicine & Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.

Original language	English
Pages (from-to)	64-73
Number of pages	10
Journal	Cell Cycle
Volume	14
Issue number	1
DOIs	https://doi.org/10.4161/15384101.2014.973337
Publication status	Published - 1 Jan 2015

Keywords

AP-1
DNA replication origin
Fos
Jun
Origin recognition complex
Transcription factor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.4161/15384101.2014.973337

Cite this

@article{e64788bfbfde4fbab26b5fa9be879e9e,

title = "DNA-protein interaction dynamics at the Lamin B2 replication origin http://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.973337",

abstract = "To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.",

keywords = "AP-1, DNA replication origin, Fos, Jun, Origin recognition complex, Transcription factor",

author = "Luca Puzzi and Laura Marchetti and Peverali, {Fiorenzo A.} and Giuseppe Biamonti and Mauro Giacca",

year = "2015",

month = jan,

day = "1",

doi = "10.4161/15384101.2014.973337",

language = "English",

volume = "14",

pages = "64--73",

journal = "Cell Cycle",

issn = "1538-4101",

publisher = "Taylor and Francis",

number = "1",

}

TY - JOUR

T1 - DNA-protein interaction dynamics at the Lamin B2 replication origin http://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.973337

AU - Puzzi, Luca

AU - Marchetti, Laura

AU - Peverali, Fiorenzo A.

AU - Biamonti, Giuseppe

AU - Giacca, Mauro

PY - 2015/1/1

Y1 - 2015/1/1

N2 - To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.

AB - To date, a complete understanding of the molecular events leading to DNA replication origin activation in mammalian cells still remains elusive. In this work, we report the results of a high resolution chromatin immunoprecipitation study to detect proteins interacting with the human Lamin B2 replication origin. In addition to the pre-RC component ORC4 and to the transcription factors USF and HOXC13, we found that 2 components of the AP-1 transcription factor, c-Fos and c-Jun, are also associated with the origin DNA during the late G1 phase of the cell cycle and that these factors interact with ORC4. Both DNA replication and AP-1 factor binding to the origin region were perturbed by cell treatment with merbarone, a topoisomerase II inhibitor, suggesting that DNA topology is essential for determining origin function.

KW - AP-1

KW - DNA replication origin

KW - Fos

KW - Jun

KW - Origin recognition complex

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=84921843637&partnerID=8YFLogxK

U2 - 10.4161/15384101.2014.973337

DO - 10.4161/15384101.2014.973337

M3 - Article

C2 - 25483070

AN - SCOPUS:84921843637

SN - 1538-4101

VL - 14

SP - 64

EP - 73

JO - Cell Cycle

JF - Cell Cycle

IS - 1

ER -

DNA-protein interaction dynamics at the Lamin B2 replication origin http://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.973337

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this