King's College London

Research portal

Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection

Research output: Contribution to journalArticle

Standard

Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. / Trivedi, Niraj; Stabley, Daniel R; Cain, Blake; Howell, Danielle; Laumonnerie, Christophe; Ramahi, Joseph S; Temirov, Jamshid; Kerekes, Ryan A; Gordon-Weeks, Phillip R; Solecki, David J.

In: Nature Communications, Vol. 8, 23.02.2017, p. 14484.

Research output: Contribution to journalArticle

Harvard

Trivedi, N, Stabley, DR, Cain, B, Howell, D, Laumonnerie, C, Ramahi, JS, Temirov, J, Kerekes, RA, Gordon-Weeks, PR & Solecki, DJ 2017, 'Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection', Nature Communications, vol. 8, pp. 14484. https://doi.org/10.1038/ncomms14484

APA

Trivedi, N., Stabley, D. R., Cain, B., Howell, D., Laumonnerie, C., Ramahi, J. S., ... Solecki, D. J. (2017). Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. Nature Communications, 8, 14484. https://doi.org/10.1038/ncomms14484

Vancouver

Trivedi N, Stabley DR, Cain B, Howell D, Laumonnerie C, Ramahi JS et al. Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. Nature Communications. 2017 Feb 23;8:14484. https://doi.org/10.1038/ncomms14484

Author

Trivedi, Niraj ; Stabley, Daniel R ; Cain, Blake ; Howell, Danielle ; Laumonnerie, Christophe ; Ramahi, Joseph S ; Temirov, Jamshid ; Kerekes, Ryan A ; Gordon-Weeks, Phillip R ; Solecki, David J. / Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. In: Nature Communications. 2017 ; Vol. 8. pp. 14484.

Bibtex Download

@article{35b92245cb7a4f74a5fedb2fa2d71428,
title = "Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection",
abstract = "Neuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration. Lattice light-sheet and structured illumination microscopy reveal a proximal leading process nanoscale architecture wherein f-actin and drebrin intervene between microtubules and the plasma membrane. Functional perturbations of drebrin demonstrate that proximal leading process microtubule-actomyosin coupling steers the direction of centrosome and somal migration, as well as the switch from tangential to radial migration. Finally, the Siah2 E3 ubiquitin ligase antagonizes drebrin function, suggesting a model for control of the microtubule-actomyosin interfaces during neuronal differentiation.",
author = "Niraj Trivedi and Stabley, {Daniel R} and Blake Cain and Danielle Howell and Christophe Laumonnerie and Ramahi, {Joseph S} and Jamshid Temirov and Kerekes, {Ryan A} and Gordon-Weeks, {Phillip R} and Solecki, {David J}",
year = "2017",
month = "2",
day = "23",
doi = "10.1038/ncomms14484",
language = "English",
volume = "8",
pages = "14484",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection

AU - Trivedi, Niraj

AU - Stabley, Daniel R

AU - Cain, Blake

AU - Howell, Danielle

AU - Laumonnerie, Christophe

AU - Ramahi, Joseph S

AU - Temirov, Jamshid

AU - Kerekes, Ryan A

AU - Gordon-Weeks, Phillip R

AU - Solecki, David J

PY - 2017/2/23

Y1 - 2017/2/23

N2 - Neuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration. Lattice light-sheet and structured illumination microscopy reveal a proximal leading process nanoscale architecture wherein f-actin and drebrin intervene between microtubules and the plasma membrane. Functional perturbations of drebrin demonstrate that proximal leading process microtubule-actomyosin coupling steers the direction of centrosome and somal migration, as well as the switch from tangential to radial migration. Finally, the Siah2 E3 ubiquitin ligase antagonizes drebrin function, suggesting a model for control of the microtubule-actomyosin interfaces during neuronal differentiation.

AB - Neuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration. Lattice light-sheet and structured illumination microscopy reveal a proximal leading process nanoscale architecture wherein f-actin and drebrin intervene between microtubules and the plasma membrane. Functional perturbations of drebrin demonstrate that proximal leading process microtubule-actomyosin coupling steers the direction of centrosome and somal migration, as well as the switch from tangential to radial migration. Finally, the Siah2 E3 ubiquitin ligase antagonizes drebrin function, suggesting a model for control of the microtubule-actomyosin interfaces during neuronal differentiation.

U2 - 10.1038/ncomms14484

DO - 10.1038/ncomms14484

M3 - Article

C2 - 28230156

VL - 8

SP - 14484

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -

View graph of relations

© 2018 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454