Drosophila rolling pebbles: A multidomain protein required for myoblast fusion that recruits D-titin in response to the myoblast attractant dumbfounded

S D Menon; W Chia

doi:10.1016/S1534-5807(01)00075-2

Drosophila rolling pebbles: A multidomain protein required for myoblast fusion that recruits D-titin in response to the myoblast attractant dumbfounded

S D Menon, W Chia

King's College London

Research output: Contribution to journal › Article › peer-review

102 Citations (Scopus)

Abstract

The fusion of myoblasts leading to the formation of myotubes is an integral part of skeletal myogenesis in many organisms. In Drosophila, specialized founder myoblasts initiate fusion through expression of the receptor-like attractant Dumbfounded (Duf), which brings them into close contact with other myoblasts. Here, we identify Rols7, a gene expressed in founders, as an essential component for fusion during myotube formation. During fusion, Rols7 localizes in a Duf-dependent manner at membrane sites that contact other myoblasts. These sites are also enriched with D-Titin, which functions to maintain myotube structure and morphology. When Rols7 is absent or its localization is perturbed, the enrichment of D-Titin fails to occur. Rols7 integrates the initial event of myoblast attraction with the downstream event of myotube structural organization by linking Duf to D-Titin.

Original language	English
Pages (from-to)	691 - 703
Number of pages	13
Journal	Developmental Cell
Volume	1
Issue number	5
DOIs	https://doi.org/10.1016/S1534-5807(01)00075-2
Publication status	Published - Nov 2001

Access to Document

10.1016/S1534-5807(01)00075-2

Cite this

@article{69bfe9d81b1e4ae49d4ed799f906ac58,

title = "Drosophila rolling pebbles: A multidomain protein required for myoblast fusion that recruits D-titin in response to the myoblast attractant dumbfounded",

abstract = "The fusion of myoblasts leading to the formation of myotubes is an integral part of skeletal myogenesis in many organisms. In Drosophila, specialized founder myoblasts initiate fusion through expression of the receptor-like attractant Dumbfounded (Duf), which brings them into close contact with other myoblasts. Here, we identify Rols7, a gene expressed in founders, as an essential component for fusion during myotube formation. During fusion, Rols7 localizes in a Duf-dependent manner at membrane sites that contact other myoblasts. These sites are also enriched with D-Titin, which functions to maintain myotube structure and morphology. When Rols7 is absent or its localization is perturbed, the enrichment of D-Titin fails to occur. Rols7 integrates the initial event of myoblast attraction with the downstream event of myotube structural organization by linking Duf to D-Titin.",

author = "Menon, {S D} and W Chia",

year = "2001",

month = nov,

doi = "10.1016/S1534-5807(01)00075-2",

language = "English",

volume = "1",

pages = "691 -- 703",

journal = "Developmental Cell",

issn = "1878-1551",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - Drosophila rolling pebbles: A multidomain protein required for myoblast fusion that recruits D-titin in response to the myoblast attractant dumbfounded

AU - Menon, S D

AU - Chia, W

PY - 2001/11

Y1 - 2001/11

N2 - The fusion of myoblasts leading to the formation of myotubes is an integral part of skeletal myogenesis in many organisms. In Drosophila, specialized founder myoblasts initiate fusion through expression of the receptor-like attractant Dumbfounded (Duf), which brings them into close contact with other myoblasts. Here, we identify Rols7, a gene expressed in founders, as an essential component for fusion during myotube formation. During fusion, Rols7 localizes in a Duf-dependent manner at membrane sites that contact other myoblasts. These sites are also enriched with D-Titin, which functions to maintain myotube structure and morphology. When Rols7 is absent or its localization is perturbed, the enrichment of D-Titin fails to occur. Rols7 integrates the initial event of myoblast attraction with the downstream event of myotube structural organization by linking Duf to D-Titin.

AB - The fusion of myoblasts leading to the formation of myotubes is an integral part of skeletal myogenesis in many organisms. In Drosophila, specialized founder myoblasts initiate fusion through expression of the receptor-like attractant Dumbfounded (Duf), which brings them into close contact with other myoblasts. Here, we identify Rols7, a gene expressed in founders, as an essential component for fusion during myotube formation. During fusion, Rols7 localizes in a Duf-dependent manner at membrane sites that contact other myoblasts. These sites are also enriched with D-Titin, which functions to maintain myotube structure and morphology. When Rols7 is absent or its localization is perturbed, the enrichment of D-Titin fails to occur. Rols7 integrates the initial event of myoblast attraction with the downstream event of myotube structural organization by linking Duf to D-Titin.

UR - http://www.scopus.com/inward/record.url?scp=0035515528&partnerID=8YFLogxK

U2 - 10.1016/S1534-5807(01)00075-2

DO - 10.1016/S1534-5807(01)00075-2

M3 - Article

SN - 1878-1551

VL - 1

SP - 691

EP - 703

JO - Developmental Cell

JF - Developmental Cell

IS - 5

ER -

Drosophila rolling pebbles: A multidomain protein required for myoblast fusion that recruits D-titin in response to the myoblast attractant dumbfounded

Abstract

Access to Document

Other files and links

Fingerprint

Cite this