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Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging

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Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging. / Stasiuk, Graeme J.; Minuzzi, Florencia; Sae-Heng, Myra; Rivas, Charlotte; Juretschke, Hans Paul; Piemonti, Lorenzo; Allegrini, Peter R.; Laurent, Didier; Duckworth, Andrew R.; Beeby, Andrew; Rutter, Guy A.; Long, Nicholas J.

In: Chemistry - A European Journal, Vol. 21, No. 13, 23.03.2015, p. 5023-5033.

Research output: Contribution to journalArticle

Harvard

Stasiuk, GJ, Minuzzi, F, Sae-Heng, M, Rivas, C, Juretschke, HP, Piemonti, L, Allegrini, PR, Laurent, D, Duckworth, AR, Beeby, A, Rutter, GA & Long, NJ 2015, 'Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging', Chemistry - A European Journal, vol. 21, no. 13, pp. 5023-5033. https://doi.org/10.1002/chem.201406008

APA

Stasiuk, G. J., Minuzzi, F., Sae-Heng, M., Rivas, C., Juretschke, H. P., Piemonti, L., Allegrini, P. R., Laurent, D., Duckworth, A. R., Beeby, A., Rutter, G. A., & Long, N. J. (2015). Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging. Chemistry - A European Journal, 21(13), 5023-5033. https://doi.org/10.1002/chem.201406008

Vancouver

Stasiuk GJ, Minuzzi F, Sae-Heng M, Rivas C, Juretschke HP, Piemonti L et al. Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging. Chemistry - A European Journal. 2015 Mar 23;21(13):5023-5033. https://doi.org/10.1002/chem.201406008

Author

Stasiuk, Graeme J. ; Minuzzi, Florencia ; Sae-Heng, Myra ; Rivas, Charlotte ; Juretschke, Hans Paul ; Piemonti, Lorenzo ; Allegrini, Peter R. ; Laurent, Didier ; Duckworth, Andrew R. ; Beeby, Andrew ; Rutter, Guy A. ; Long, Nicholas J. / Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging. In: Chemistry - A European Journal. 2015 ; Vol. 21, No. 13. pp. 5023-5033.

Bibtex Download

@article{4beb3e55c7e34fdfbe9a3528e417446f,
title = "Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging",
abstract = "Despite the contribution of changes in pancreatic β-cell mass to the development of all forms of diabetes mellitus, few robust approaches currently exist to monitor these changes prospectively in vivo. Although magnetic-resonance imaging (MRI) provides a potentially useful technique, targeting MRI-active probes to the β cell has proved challenging. Zinc ions are highly concentrated in the secretory granule, but they are relatively less abundant in the exocrine pancreas and in other tissues. We have therefore developed functional dual-modal probes based on transition-metal chelates capable of binding zinc. The first of these, Gd·1, binds ZnII directly by means of an amidoquinoline moiety (AQA), thus causing a large ratiometric Stokes shift in the fluorescence from λem=410 to 500 nm with an increase in relaxivity from r1=4.2 up to 4.9 mM-1s-1. The probe is efficiently accumulated into secretory granules in β-cell-derived lines and isolated islets, but more poorly by non-endocrine cells, and leads to a reduction in T1 in human islets. In vivo murine studies of Gd·1 have shown accumulation of the probe in the pancreas with increased signal intensity over 140 minutes.",
keywords = "diabetes, fluorescence, imaging agents, lanthanides, zinc",
author = "Stasiuk, {Graeme J.} and Florencia Minuzzi and Myra Sae-Heng and Charlotte Rivas and Juretschke, {Hans Paul} and Lorenzo Piemonti and Allegrini, {Peter R.} and Didier Laurent and Duckworth, {Andrew R.} and Andrew Beeby and Rutter, {Guy A.} and Long, {Nicholas J.}",
year = "2015",
month = mar,
day = "23",
doi = "10.1002/chem.201406008",
language = "English",
volume = "21",
pages = "5023--5033",
journal = "Chemistry (Weinheim an der Bergstrasse, Germany)",
issn = "0947-6539",
number = "13",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Dual-Modal Magnetic Resonance/Fluorescent Zinc Probes for Pancreatic β-Cell Mass Imaging

AU - Stasiuk, Graeme J.

AU - Minuzzi, Florencia

AU - Sae-Heng, Myra

AU - Rivas, Charlotte

AU - Juretschke, Hans Paul

AU - Piemonti, Lorenzo

AU - Allegrini, Peter R.

AU - Laurent, Didier

AU - Duckworth, Andrew R.

AU - Beeby, Andrew

AU - Rutter, Guy A.

AU - Long, Nicholas J.

PY - 2015/3/23

Y1 - 2015/3/23

N2 - Despite the contribution of changes in pancreatic β-cell mass to the development of all forms of diabetes mellitus, few robust approaches currently exist to monitor these changes prospectively in vivo. Although magnetic-resonance imaging (MRI) provides a potentially useful technique, targeting MRI-active probes to the β cell has proved challenging. Zinc ions are highly concentrated in the secretory granule, but they are relatively less abundant in the exocrine pancreas and in other tissues. We have therefore developed functional dual-modal probes based on transition-metal chelates capable of binding zinc. The first of these, Gd·1, binds ZnII directly by means of an amidoquinoline moiety (AQA), thus causing a large ratiometric Stokes shift in the fluorescence from λem=410 to 500 nm with an increase in relaxivity from r1=4.2 up to 4.9 mM-1s-1. The probe is efficiently accumulated into secretory granules in β-cell-derived lines and isolated islets, but more poorly by non-endocrine cells, and leads to a reduction in T1 in human islets. In vivo murine studies of Gd·1 have shown accumulation of the probe in the pancreas with increased signal intensity over 140 minutes.

AB - Despite the contribution of changes in pancreatic β-cell mass to the development of all forms of diabetes mellitus, few robust approaches currently exist to monitor these changes prospectively in vivo. Although magnetic-resonance imaging (MRI) provides a potentially useful technique, targeting MRI-active probes to the β cell has proved challenging. Zinc ions are highly concentrated in the secretory granule, but they are relatively less abundant in the exocrine pancreas and in other tissues. We have therefore developed functional dual-modal probes based on transition-metal chelates capable of binding zinc. The first of these, Gd·1, binds ZnII directly by means of an amidoquinoline moiety (AQA), thus causing a large ratiometric Stokes shift in the fluorescence from λem=410 to 500 nm with an increase in relaxivity from r1=4.2 up to 4.9 mM-1s-1. The probe is efficiently accumulated into secretory granules in β-cell-derived lines and isolated islets, but more poorly by non-endocrine cells, and leads to a reduction in T1 in human islets. In vivo murine studies of Gd·1 have shown accumulation of the probe in the pancreas with increased signal intensity over 140 minutes.

KW - diabetes

KW - fluorescence

KW - imaging agents

KW - lanthanides

KW - zinc

UR - http://www.scopus.com/inward/record.url?scp=84924611992&partnerID=8YFLogxK

U2 - 10.1002/chem.201406008

DO - 10.1002/chem.201406008

M3 - Article

C2 - 25736590

AN - SCOPUS:84924611992

VL - 21

SP - 5023

EP - 5033

JO - Chemistry (Weinheim an der Bergstrasse, Germany)

JF - Chemistry (Weinheim an der Bergstrasse, Germany)

SN - 0947-6539

IS - 13

ER -

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