Dynamic and differential regulation of NKCC1 by calcium and cAMP in the native human colonic epithelium

Amy Reynolds, Alyson Parris, Luke A. Evans, Susanne Lindqvist, Paul Sharp, Michael Lewis, Richard Tighe, Mark R. Williams*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

The capacity of the intestine to secrete fluid is dependent on the basolateral Na+-K+-2Cl(-) co-transporter (NKCC1). Given that cAMP and Ca2+ signals promote sustained and transient episodes of fluid secretion, respectively, this study investigated the differential regulation of functional NKCC1 membrane expression in the native human colonic epithelium. Tissue sections and colonic crypts were obtained from sigmoid rectal biopsy tissue samples. Cellular location of NKCC1, Na+-K+-ATPase, M3 muscarinic acetylcholine receptor (M(3)AChR) and lysosomes was examined by immunolabelling techniques. NKCC1 activity (i.e. bumetanide-sensitive NH4 (+) uptake), intracellular Ca2+ and cell volume were assessed by 2',7'-bis(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF), Fura-2 and differential interference contrast/calcein imaging. Unstimulated NKCC1 was expressed on basolateral membranes and exhibited a topological expression gradient, predominant at the crypt base. Cholinergic Ca2+ signals initiated at the crypt base and spread along the crypt axis. In response, NKCC1 underwent a Ca2+-dependent 4 h cycle of recruitment to basolateral membranes, activation, internalization, degradation and re-expression. Internalization was prevented by the epidermal growth factor receptor kinase inhibitor tyrphostin-AG1478, and re-expression was prohibited by the protein synthesis inhibitor cylcoheximide; the lysosome inhibitor chloroquine promoted accumulation of NKCC1 vesicles. NKCC1 internalization and re-expression were accompanied by secretory volume decrease and bumetanide-sensitive regulatory volume increase, respectively. In contrast, forskolin (i.e. cAMP elevation)-stimulated NKCC1 activity was sustained, and membrane expression and cell volume remained constant. Co-stimulation with forskolin and acetylcholine promoted dramatic recruitment of NKCC1 to basolateral membranes and prolonged the cycle of co-transporter activation, internalization and re-expression. In conclusion, persistent NKCC1 activation by cAMP is constrained by a Ca2+-dependent cycle of co-transporter internalization, degradation and re-expression; this is a novel mechanism to limit intestinal fluid loss.

Original languageEnglish
Pages (from-to)507-524
Number of pages18
JournalThe Journal of Physiology
Volume582
Issue number2
DOIs
Publication statusPublished - 15 Jul 2007

Keywords

  • EPIDERMAL-GROWTH-FACTOR
  • ACTIVATED PROTEIN-KINASE
  • TRANSMEMBRANE CONDUCTANCE REGULATOR
  • NA+-K+-2CL(-) COTRANSPORTER NKCC1
  • STIMULATED CHLORIDE SECRETION
  • K-CL COTRANSPORTER
  • FACTOR RECEPTOR
  • ION-TRANSPORT
  • SURFACE EXPRESSION
  • MOLECULAR-BASIS

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